CD160在慢性淋巴细胞白血病中的生物学特征研究

Biological Characteristics of CD160 in Patients with Chronic Lymphocytic Leukemia

目的探讨CD160在慢性淋巴细胞白血病中的表达及临床意义。方法应用流式细胞仪检测22名健康对照者、91例CLL患者、76例B细胞非霍奇金淋巴瘤CD160的表达。同时检测91例CLL患者和22名对照者PTEN、p-Akt的表达。结果22名健康对照1例(4.5%)CD160呈弱阳性。91例CLL患者89例(97.80%)CD160阳性,远高于对照组,差异有统计学意义(χ2=89.38,P<0.01)。CLL患者PTEN蛋白较对照组表达明显减低,p-Akt表达较对照组显著增高,且CLL患者CD160表达水平与p-Akt表达水平呈正相关,而与PTEN表达水平呈负相关。76例淋巴瘤患者5例(6.58%)CD160阳性,分别为4例HCL,1例SMZL。48例MCL患者CD160均阴性。CD160在CLL中的阳性率远高于淋巴瘤,差异有统计学意义(χ2=140.06,P<0.01)。CLL与MCL免疫表型差异主要是CD23与FMC7的阳性率不同,CD160在CLL中的阳性率远高于MCL,差异有统计学意义(χ2=130.51,P<0.01)。四格表诊断性试验分析显示,CD160在CLL中的敏感度为97.80%,特异性为90.79%,准确度为94.61%。CD160在CLL患者高表达与临床预后无关。结论 CD160在CLL细胞中普遍高表达,有助于CLL的诊断和鉴别诊断。

Objective To investigate the expression of CD160 in chronic lymphocytic leukemia （CLL） and its clinical signification.Methods Twenty-two healthy control,91 cases of CLL and 76 cases of B cell non hedgkin limphoma （NHL） were detected for CD160 expression by flow cytometry,and PTEN and p-Akt were also analyzed from CLL patients and control by flow cytometry.Results Most of CLL patients expressed CD160 positively （89/91,97.80％）,compared with healthy control with CD160 expression weakly positive in only one person （1/22,4.5％）,and the positive rate between two group had significant differences （x2 =89.38,P ＜ 0.01）.In the same time,we detected a decrease in PTEN protein accompanied by an increase in phospho-AKT in CLL patients,and data showed that CD160 expression positively correlated with phospho-AKT and negatively correlated with PTEN protein expression.In lymphoma group,5/76 （6.58％） patients expressed CD160 positively,that included in 4 cases of HCL and 1case of splenic marginal zone lymphoma （SMZL）.All patients of mantle cell lymphoma （MCL） were CD160 negative.The positive rate of CD160 expression were significantly higher in CLL patients than in lymphoma patients （x2 =140.06,P ＜ 0.01）.Apart from CD23 and FMC7 differentially expressed in CLL and MCL,significant differences （x2 =130.51,P ＜0.01） of CD160 expression were observed in the two groups.Diagnostic test by fourfold table analysis showed that CD160 expression sensitivity in CLL was 97.80％,specificity 90.79％ and 94.61％ accuracy.CD160 high expression in CLL patients was not associated with clinical prognosis in this study.Concluson The higher CD160 expression generally in CLL cells would be helpful for the diagnosis and differential diagnosis of CLL.