人脐带血间充质干细胞对人胃癌MGC80-3细胞增殖的影响

Effect of human umbilical cord blood mesenchymal stem cells on proliferation of gastric carinoma MGC80-3 cell

目的探讨人脐带血间充质干细胞(h UCB-MSCs)对人胃癌MGC80-3细胞体外增殖的影响。方法原代培养h UCBMSCs,通过流式细胞仪进行细胞表型鉴定;采用MTT法测定不同浓度h UCB-MSCs条件培养基作用于胃癌MGC80-3细胞后,胃癌细胞24 h、48 h、72 h的抑制率;倒置相差显微镜观察胃癌MGC80-3细胞形态的变化;流式细胞技术测定胃癌MGC80-3细胞凋亡率。结果采用流式细胞仪表型分析获得的第4代h UCB-MSCs均质性好;MTT法显示,不同浓度h UCB-MSCs条件培养基处理MGC80-3细胞24 h、48 h、72 h后,各实验组抑制率均有不同程度升高;倒置相差显微镜观察显示细胞形态发生变化;流式细胞术分析结果显示,MSC-CM能诱导MGC80-3细胞发生凋亡,细胞凋亡率随着MSC-CM的升高而升高,各实验组与空白对照组比较及各实验组之间比较,差异均有统计学意义(P<0.05)。结论 h UCB-MSCs能明显抑制人胃癌细胞株MGC80-3细胞的体外增殖与转移,且作用效果具有剂量和时间依赖性。

Objective To investigate the effect of human umbilical cord blood mesenchymal stem cells （hUCB-MSCs） on proliferation of gastric carcinoma MGC80-3 cell.Methods hUCB-MSCs were primary cultured in vitro and identified the phenotype by the flow cytometry.Different concentrations of hUCB-MSCs condition medium were added into the human gastric carcinoma MGC80-3 cell and the inhibition efficiency was evaluated by MTT at 24 hours,48 hours and 72 hours.The change of gastric carcinoma MGC80-3 cells morphology was observed by inverted microscope.The rate of gastric carcinoma cell MGC80-3 apoptosis was evaluated by flow cytometry.Results Phenotype identification of MSC obtained by flow cytometry analysis showed that the fourth generation of MSC had good homogeneity.The results of MTT showed that：MGC80-3 cells was treated with different concentration hUCB-MSCs after 24 hours,48 hours,72 hours,the inhibition increased with different degree in each experimental group.The observation of inverted microscope found the morphology of MGC80-3 cells were different.The results of flow cytometry showed that：MSC-CM could induce apoptosis of MGC 80-3,the rate of cell apoptosis increased with the increasing of concentration,and there were significantly difference between experimental groups and control group and among experimental groups （P ＜ 0.05）.Conclusion hUCB-MSCs can obviously inhibit the proliferation and metastasis of human gastric carcinoma MGC80-3 cells,and the effect is in a dose and time-dependent manner.