以铁皮石斛花、叶配伍的和胃茶对胃肠运动的影响

The Effects of Hewei Tea on Gastrointestinal Motility

目的通过在体实验胃排空和小肠推进实验及离体实验离体肠管的张力、振幅、频率,探究一种含铁皮石斛叶和花的和胃茶煎剂对胃肠功能的影响。方法在体实验:将小鼠随机分为正常对照组、香砂六君丸组、和胃茶饮用组、和胃茶灌胃组,分别灌服生理盐水20mL·kg-1,香砂六君丸混悬液2.33g·kg-1,生理盐水20mL·kg-1,和胃茶煎剂0.72g·kg-1,连续灌胃14d后,禁食不禁水12h,以营养性半固体糊灌胃,测定小鼠胃排空率和小肠推进率。离体实验:取SD大鼠离体小肠,按离体肠平滑肌灌注实验方法,观察在正常台氏液条件下加入不同剂量的和胃茶浸提物(浴槽内终浓度为0.0185、0.0370、0.0740、0.1480g·mL-1)后大鼠肠平滑肌的收缩状况及以生理盐水和香砂六君丸水煎液作为对照,在乙酰胆碱作用下加入和胃茶浸提物后大鼠肠平滑肌的收缩状况。结果在体实验:和胃茶灌胃组小鼠胃排空率明显降低(P<0.01)。各组小肠推进率两两比较,差异无统计学意义(P>0.05)。离体实验:和胃茶煎剂对大鼠离体小肠运动呈明显抑制作用,表现为振幅减弱,张力下降,频率减慢并能拮抗氯化乙酰胆碱引起的肠平滑肌收缩活动。结论和胃茶煎剂对胃肠运动有较明显抑制作用,能解除肠痉挛。

Objective To explore the effects of Hewei Tea, a composition of Chinese herbs containing leaves and flowers of Dendrobium officinale, on gastrointestinal function by means of the gastric emptying and small intestine propulsion experiments in vivo, and the tension, amplitude and frequency of isolated intestine. Methods In vivo experiments：Mice were randomly divided into control group, Xiangshaliujun pill group, Hewei Tea drinking group and Hewei Tea lavage group, and treated by a gavage of normal saline（20mL·kg-1）, suspension of Xiangshaliujun pill（2.33g·kg^-1）, normal saline（20mL· kg^-1）, and decoction of Hewei Tea（0.72 g·kg^-1）, once a day, respectively. After 14 days, the mouse were given by gavage with nutritional semisolid paste to measure the rates of gastric emptying and small intestinal propulsion in mice, after deprived of food but not water for 12 hours. In vitro experiments： According to the isolated small intestinal smooth muscle perfusion experiment method, we took the small intestine isolated from SD rats to observed changes of intestinal smooth muscle contraction after adding different doses of Hewei Tea decoction （final concentration in bath was 0.0185、 0.0370、 0.0740、 0.1480g·mL^ -1） in normal Tyrode’s solution or in the condition of acetylcholine, compared with normal saline and Xiangshaliujiu pill decoction. Results In vivo experiments： Gastric emptying rate of Hewei Tea lavage group significantly reduced（P〈0.01）. The pairwise comparison of small intestine propulsion of groups, there was no significant difference （P〉0.05）. In vitro experiments： Hewei Tea decoction significantly inhibited intestinal motility of rats with reducing the amplitude, declining tension, slowing down the frequency, and antagonized the contraction of intestinal smooth muscle induced by acetylcholine. Conclusion Hewei Tea decoction has obvious inhibitory effects on gastrointestinal movement, which can relieve intestinal cramps.