Bcl-2与Ki-67蛋白在喉鳞癌中的表达检测及临床相关性分析

Expression and Analysis of Correlation of Bcl-2 and Ki-67 in Laryngeal Squamous Cell Carcinoma

目的 检测喉鳞状细胞癌中Bcl-2蛋白和Ki-67蛋白的表达水平并分析两者的相关性. 方法 收集54例喉鳞癌组织和42例正常喉粘膜组织的样本,采用SP免疫组化法观察石蜡切片中Bcl-2、Ki-67蛋白的表达,并结合临床相关病理因素分析在喉鳞状细胞癌中两者的相关性. 结果 在喉鳞癌组织中,Bcl-2蛋白高表达,阳性表达率70.37％（38/54）,正常的喉粘膜组织中Bcl-2蛋白阳性微弱表达或不表达,阳性率9.5％ （4/42）（P＜0.05）;Bcl-2蛋白表达水平与临床分期、淋巴结转移有明显相关性：在晚期（Ⅲ～Ⅳ期）的表达高于早期（Ⅰ～Ⅱ期）（P＜0.05）,而其在不同年龄、性别中的表达,差异无统计学意义（P＞0.05）.Ki-67蛋白在喉鳞状细胞癌中的表达为81.48％（44/54）,在正常组织中表达明显下调仅为16.67％ （7/42）;在喉鳞癌中Bcl-2蛋白与Ki-67蛋白的表达呈正相关（rs=0.317,P＜0.05）. 结论 与正常组织比较,在喉鳞癌中Bcl-2蛋白与Ki-67蛋白表达明显上调,并呈正相关,可能两者在喉鳞癌癌变中发挥了协同作用.

Objective The expression of Bcl-2 and Ki-67 was detected in human laryngeal squamous cell carcinoma （I_SCC） and its correlation was analyzed. Methods The immunohistochemical method SP was used to analyze the expression and clinical significance of Bcl-2 and Ki-67 in 54 cases of LSCC and 42 cases of the normal laryngeal mucosa as control and the correlation between the two groups. Results In laryngeal squamous carcinoma, the Bcl-2 protein positive overexpression rate was 70.37% （38/54） ,while it was weak or had no expression in the normal laryngeal mucosa （P 〈 0. 05 ）. The Bcl-2 protein expression level was obviously correlated with clinical stages and lymph node metastasis. Moreo- ver, overexpression of Bcl-2 was observed more frequently in LSCC with late TNM stsges （ Ⅲ-Ⅳ ） （P 〈 0. 05 ）. There was no significant difference in the positive expression of Bcl-2 among gender, age and pathological type （ P 〉 0.05 ）. Nuclear antigen Ki-67 was expressed in 81.48% （44/54） of patients with LSCC. The expression in normal laryngeal mucosa was significantly lower only for 16.67% （7/42） （P 〈 0.05）. The expression of Ki-67 in LSCC was positively related to the expressions of Bcl-2 （r = 0. 317,P 〈 0.05 ）. Couclusions Compared with laryngeal normal tissue, Bcl-2 and Ki-67 pro- tein expression significantly raised in laryngeal squamous cell carcinoma and were positively correlated with each other, which could both play a collaborative role in the carcinogenesis of laryngeal squamous cell carcinoma.