摘要
目的检测miR-26a在卵巢癌组织的表达改变,明确miR-26a调控卵巢癌细胞生长的分子机制。方法运用qRT-PCR检测52例卵巢癌及相应癌旁组织中miR-26a的表达改变;将miR-26a mimics转染卵巢癌细胞CAOV3,以MTDH siRNA为阳性对照,采用Western blot检测其对MTDH蛋白表达水平的影响;然后采用MTT法检测高表达miR-26a对CAOV3细胞生长增殖的影响。结果 qRT-PCR检测结果显示,miR-26a在52例卵巢癌组织中表达下调;Western blot结果显示,过表达miR-26a或干扰MTDH可抑制MTDH蛋白的表达。MTT检测发现,过表达miR-26a或干扰MTDH可抑制人CAOV3细胞的生长(P<0.05)。结论 miR-26a通过调节MTDH基因的表达而抑制卵巢癌细胞的生长。
Objective To explicit the expression and role of miR-26a in ovarian cancer tissues and cells. Meth- ods qRT-PCR was used to detect the expression of miR-26a in ovarian cancers and normal ovarian tissues. CAOV3 ceils were transfeeted with miR-26a mimics, and MTDH siRNA as for positive control, then Western blotting was performed to de- tect the expression of MTDH protein. The proliferation ability of CAOV3 cells was evaluated by MTT assy. Results miR- 26a was down-regulated in ovarian cancer tissues. Western blot showed that the expressions of MTDH protein was inhibited by restored miR-26a or siRNA MTDH in CAOV3 cells. Overexpression of miR-26a or siRNA MTDH inhibited the prolifera- tion of CAOV3 cells. Conclusion miR-26a suppresses cell proliferation by regulating MTDH in ovarian cancer.
出处
《中南医学科学杂志》
CAS
2014年第6期563-566,共4页
Medical Science Journal of Central South China
