广西百色地区结核分枝杆菌利福平耐药基因rpoB的突变特征分析

Characteristics of rpoB gene mutation of rifampin-resistant Mycobacterium tuberculos in Baise city,Guangxi province

目的分析百色地区结核分枝杆菌(MTB)利福平耐药相关基因rpoB的突变特征。方法收集128例结核病患者痰液标本,采用改良罗氏培养基分离培养结核分枝杆菌,绝对浓度法检测利福平耐药性,PCR技术检测耐药结核分枝杆菌rpoB基因序列并测序分析利福平耐药相关基因rpoB的突变特征。结果分离培养获得98例MTB,98例MTB出现36株利福平耐药,耐药率为36.73%,36株耐利福平菌株中有27株rpoB基因发生突变,基因突变率为75.00%,其中516位点突变率为3.70%(1/27),531位点突变率为59.26%(16/27),526位点突变率为29.63%(8/27),533位点突变率为7.41%(2/27)。突变热点依次为rpoB531、rpoB526和rpoB533。结论百色地区耐利福平结核分枝杆菌耐药性产生的主要分子机制是因为rpoB的基因发生突变。

Objective To analyze rpoB gene mutation characteristics in rifampin-resistant related Myco-bacterium tuberculosis （MTB）in Baise city. Methods The sputum specimens were collected from 128 pa-tients with tuberculosis and then were cultured on modifieded Lowenstein-Jensen culture medium,the drug sensitivity of rifampin was detected by absolute concentration method,mutation patterns of rpoB genes were detected by PCR and sequencing. Results Ninety-eight MTB isolates were obtained from 128 MTB clinical strains,36.73% （36/98）isolates were resistant to rifampin,27 of 36 rifampin-resistance MTB （75.00%）i-solates carried mutations in the rpoB gene,and 3.70% （1/27）isolates carried mutations in 516 site,59.26%（16/27）isolates carried mutations in 531 site,7.41% （2/27）isolates carried mutations in 533 site.The top 3 loci with high mutation frequency were rpoB531,rpoB526 and rpoB533. Conclusion The main mechanism of rifampin-resistant Mycobacterium tuberculos occurrence is caused by rpoB gene mutation in Mycobacterium tuberculosis in Baise city.