油樟叶提取物对人肝癌BEL-7402细胞增殖的抑制作用

Effects of the Extracts of Cinnamomum longepaniculatum Leaves on the Proliferation of Human Hepatocarcinoma Cell Strain BEL-7402

采用水蒸汽蒸馏和系统溶剂提取获得油樟叶的不同提取物,运用MTT法考查油樟叶各提取物对人肝癌BEL-7402细胞增殖抑制作用的影响。结果表明,油樟叶各提取物对BEL-7402细胞的增殖具有不同程度的抑制作用,呈现明显的量效关系;各提取物抑制作用的大小顺序为:乙酸乙酯萃取物>石油醚萃取物>乙醇提取物>1,8-桉叶油素>油樟叶精油>正丁醇萃取物;低浓度时1,8-桉叶油素与油樟叶精油的差异有统计学意义(P<0.05);高浓度时油樟叶精油、乙醇提取物、石油醚萃取物和乙酸乙酯萃取物之间的差异无统计学意义(P>0.05)。油樟叶提取物有一定抑制肝癌细胞增殖的作用,油樟叶精油的主要活性成分可能是1,8-桉叶油素,去精油后残渣的活性组分存在于石油醚部分和乙酸乙酯部分。

The extracts of Cinnamomum longepaniculatum leaves were extracted by the technique of steam distillation and solvent-refining. The effects of different extracts of C. longepaniculatum on the proliferation of human hepatocarcinoma cell strain BEL-7402 were investigated by a method of MTT. The results shown that different extracts of C. longepaniculatum leaves exihibited different degree of inhibition on the proliferation of BEL-7402 cells in a dose-dependent manner. The order of inhibition was acetic ether extract〉petroleum ether extract〉ethanol extract〉1,8-cineole〉leaves essential oil〉n-butanol extract. The difference of inhibitive activity between 1,8-cineole and leaves essential oil was statistical significance at low concentration （P〈0.05）, while no statistical significance differences were observed among leaves essential oil, ethanol extract, petroleum ether extract and acetic ether extract at hight concentration（P〉0.05）. The investigaitons indicated that the extracts of C. longepaniculatum leaves can inhibit the proliferation of human hepatocarcinoma cell, and 1,8-cineole may be act the major role in the leaves essential oil, the active ingredient of C. longepaniculatum leaves may be in the part of petroleum ether extract and acetic ether extract.