野生苦菜体外抗氧化及抑制肿瘤活性研究

The Antioxidant and Tumor Inhibiting Activity of Wild Ixeris Sonchifolia

以野生苦菜为原料,采用1,1-二苯基-2-三硝基苯肼(DPPH)清除能力、2,2-联氮-二(3-乙基-苯并噻唑-6-磺酸)二铵盐(ABTS)清除能力、羟基自由基清除能力和铁离子还原能力法评定其抗氧化能力,采用3-(4,5-二甲基噻唑-2)-2,5-二苯基四氮唑溴盐(MTT)法评定苦菜提取物对雌激素依赖性人类乳腺癌细胞MCF-7和雌激素非依赖性人乳腺癌细胞MDA-MB-231的抑制活性。结果表明,苦菜甲醇提取物对ABTS、DPPH都具有较强的清除能力,且具有较强的铁离子还原能力,对雌激素依赖性人类乳腺癌细胞MCF-7和雌激素非依赖性人乳腺癌细胞MDA-MB-231均有显著的抑制活性。苦菜的甲醇提取物对MCF-7和MDA-MB-231半抑制率分别为99、107μg/mL。

The wild Ixeris Sonchifolia （from Shanxi, China） was used to evaluate its antioxidant activity and tumor inhibiting activity. The antioxidant activity was evaluated using the 2, 2-diphenyl-1-picrylhydracyl （DPPH＆#183;）, hydroxyl free radical （OH＆#183;） and 2,2＇-azinobis （3-ethylbenzothiazoline-6-sulfonicacid） （ABTS＆#183;＋） scavenging methods and the ferric reducing antioxidant power assay. 3-（4,5-dimethylthiazol-2-yl）-2,5-diphenyltetrazolium bromide （MTT） assay was employed to evaluate its tumor inhibiting activity. The results showed that the Ixeris Sonchifolia extracts had excellent DPPH＆#183;, ABTS＆#183;＋scavenging capacity and reducing power. The Ixeris Sonchifolia extracts can significantly inhibit human breast tumor MDA-MB231 （estrogen-independent tumor） and MCF-7 （estrogen-dependent tumor）. The IC50 （half maximal inhibitory concentration） of MDA-MB231 and MCF-7 were 99 and 107μg/mL, respectively.