黄芪甲苷对肺纤维化大鼠的治疗作用及对肺组织中血清和糖皮质激素调节蛋白激酶1表达的影响

Efficacy of Astragaloside for the Treatment of Pulmonary Fibrosis in Rats and Its Influence on the Expression of Serum and Glucocorticoid-regulated Proteinkinase-1 in Lung Tissue

【目的】研究黄芪甲苷对肺纤维化（PF ）大鼠的治疗作用及对肺组织中血清和糖皮质激素调节蛋白激酶1（SGK1）表达的影响。【方法】36只SD大鼠，随机分为对照组、模型组和黄芪甲苷干预组。模型组和干预组气管内注射博来霉素（BLM ，5mg／kg）诱导PF ，对照组在相同条件下给予生理盐水。干预组大鼠d2开始每天经胃管灌服0．1g／L黄芪甲苷2mL ，其余两组相同条件下给予生理盐水。治疗的d7和d28处死动物取出肺组织，进行病理学观察；用免疫组化和RT‐PCR检测各组鼠肺组织SGK1蛋白及mRNA表达的水平。【结果】病理学观察显示：与模型组比较，干预组肺泡炎和纤维化程度均减轻，SGK1蛋白及mRNA表达显著降低；与对照组比较，干预组存在一定程度肺泡炎和纤维化，SGK1蛋白及mRNA表达明显高于对照组。【结论】黄芪甲苷可能通过抑制SGK1的过度表达，对实验性大鼠PF具有良好的治疗作用。

[Objective] To explore the efficacy of Astragaloside for the treatment of pulmonary fibrosis （PF） in rats and its influence on the expression of serum and glucocorticoid regulated proteinkinase 1（SGK1） .[Methods] A total of 36 male SD rats were randomly divided into control group ,model group and Astragalo‐side intervention group .The model group and treatment group were induced to produce PF by endotracheal in‐jection of bleomycin ,while the control group was given normal saline in the same condition .From the second day ,the rats in intervention group were intragastric infusion with 0 .1g/L Astragaloside 2ml ,while the rats in other two groups were given normal saline in the same condition .The rats were sacrificed at d7 and d28 of treatment ,and lung tissues were collected for pathological observation .Immunohistochemical method and RT‐PCR were used to detect the expression levels of serum and SGK 1 in lung tissue of rats of each group .[Re‐sults] Pathological examination showed that the degree of alveolitis and pulmonary fibrosis in intervention group was significantly reduced in comparison with the model group ,and the expressions of serum and SGK1 in intervention group were obviously higher than those in control group .[Conclusion] Astragalaside may have good efficacy in treating experimental rats with PF through inhibiting the over expression of serum and SGK 1 .