不同小麦基因型对γ射线辐照敏感性的分子解析

Molecular Characterization of Radiation Sensitivity of Different Wheat Genotypes Irradiated by γ Rays

本研究以63份小麦品种(系)的风干种子为材料,分别利用0 Gy、100 Gy、150 Gy和250 Gy剂量的60Coγ射线辐照,通过种子发芽试验和基因表达等方法,探讨不同小麦基因型间辐射敏感性差异及辐射敏感性相关基因的表达模式。结果表明:根据苗高损伤效应,将63份不同小麦基因型分为敏感型(核优1号、中优206和太原703等)、较敏感型(旱选10号、济麦20和中麦175等)、较钝感型(德抗961、豫麦68和淮麦20等)和钝感型(衡观136、邯6172和偃展4110等)4类。所选材料γ射线辐照后,敏感型基因型中Ta Ku70和Ta Ku80基因诱导表达明显,钝感型基因型中Ta Ku70和Ta Ku80基因诱导表达不明显。本研究表明不同小麦基因型间辐射敏感性差异明显且与Ta Ku70和Ta Ku80基因表达模式有关。

In this paper,sixty three wheat genotypes irradiated by γ rays with the doses of 0 Gy,100 Gy,150 Gy and 250 Gy,respectively were used to investigate the difference of radiation sensitivity and its molecular mechanism through germination test and gene expression analysis.The results showed that based on the damage effect of seedling height,sixty three wheat genotypes could be divided into four categories：i.e.,the sensitive（Heyou 1,Zhongyou206 and Taiyuan 703 et al）,the intermediate sensitive（Hanxuan No.10,Jimai 20 and Zhongmai 175 et al）,the intermediate insensitive（Dekang 961,Yumai 68 and Huaimai 20 et al） and the insensitive（Hengguan 136,Han 6172 and Yanzhan 4110 et al）.The analysis of expression pattern of the TaKu70 and TaKu80 gene responsed to the γ ray irradiation revealed that there were significant differences between the induced expression amounts of the control group and those of treatment group among the sensitive genotypes.While there were no significant differences in the insensitive genotypes.It was concluded that the radiation sensitivity was significantly different in the sixty three wheat genotypes,and the differences upon radiation sensitivity were significantly associated with expression pattern of the TaKu70 and TaKu80 gene responsed to the γ rays.