摘要
用0.15%秋水仙素附加2.00%二甲基亚砜诱变离体培养的卷丹小鳞茎,避光条件下摇床诱导,用组织培养结合不定芽诱导技术获得了多倍体苗,并对多倍体染色体数目进行鉴定。结果显示,诱导96 h效果最好,变异率达到54.29%。细胞学观察发现,对照为三倍体、非整倍体和极少数单倍体细胞组成的嵌合体,诱变出的4棵变异株细胞分别为染色体数目由53~72条的不同比例构成,属于典型的非整倍的异倍型嵌合体。诱变株与对照植株间幼苗叶形指数、气孔密度及气孔大小等特征差异比较明显。
Lilium lancifolium bulblets were treated by 0.15% colchicine added with 2.00% dimethyl sulfoxide in vitro without light,and the polyploid seedlings were obtained by adventitious bud induction technology.The results of the chromosome quantity indicated that 96 h was the best induction time with the mutation rate of 54.29%.Via the cytological observation,as the control group was consisted by chimera including triploid,aneuploid,and few haploid,the chromosome quantity of these 4 mutative strains was consisted by the different proportion of 53-72 chromosome cells,and they were obviously aneuploid cytochimera.The mutant group showed obvious difference from the control group in leaf shape index of the seedling,stoma density,and stoma size.
出处
《植物遗传资源学报》
CAS
CSCD
北大核心
2014年第6期1385-1389,共5页
Journal of Plant Genetic Resources
基金
重庆市科委直通车项目(2011BB1001)
长江师范学院科研创新团队建设计划资助项目(2014XJTD06)
关键词
卷丹
多倍体
秋水仙素
染色体
嵌合体
Lilium lancifolium
polyploid
colchicines
chromosome
chimera