多民族麻醉手术患者血浆丙泊酚浓度的测定及分析

Determination and analysis of Propofol in anesthesia patients' plasma of multiple nations

目的建立高效液相色谱法测定人血浆中丙泊酚药物浓度的方法 ,初步探究丙泊酚在不同民族的麻醉手术患者体内的药物代谢动力学,为丙泊酚的群体药物代谢动力学研究奠定基础。方法收集新疆医科大学第三附属医院麻醉科于2014年3-7月美国麻醉师协会（ASA）评级为Ⅰ-Ⅱ级的择期手术患者95例,按民族不同分成三组,汉族组54例,维吾尔族组32例,哈萨克族组9例,均静脉注射丙泊酚2 mg/kg后于0、1.5、2、2.5、3、3.5、4、5、6、8、10、15、20、30、40、50、60、70、80、90、120、150、180 min随机选择3个时间点,桡动脉取血。对血样进行预处理后用高效液相-紫外法检测血药浓度。结果采用稀疏采样在不同时间点共获取了285个浓度数据。对数据进行简单的药物代谢动力学分析,未见民族间有明显差异。结论本研究建立的方法灵敏、准确、简单,可成功应用于麻醉手术患者丙泊酚的血药浓度监测和群体药动学研究。探究民族因素对丙泊酚的药物代谢动力学的影响,还需进一步研究分析。

Objective To develop a high performance liquid chromatography method for the determination of Propofol in human plasma and to preliminary study the pharmacokinetic profile of Propofol in patients of multiple nations, lay the foundation for population pharmacokinetic studies of Propofol. Methods Totally 95 ASA Ⅰ- Ⅱ patients were collected from Department of Anesthesiology in the Third Affiliated Hospital of Xinjiang Medical University, collection time was March to July in 2014. All patients undergoing elective gastro-intestinal and intracranial surgery were studied. The patients were divided into three groups according to their nations. The Han group had 54 patients, the Uygur group had 32 patients, the Kazak group had 9 patients. All patients were induced with Propofol 2.0 mg/kg, and blood samples were taken from radial artery, free to choose three time after the end of the Propofol injection at the following time points： 0, 1.5, 2, 2.5, 3, 3.5, 4, 5, 6, 8, 10, 15, 20, 30, 40, 50, 60, 70, 80, 90, 120, 150, 180 min. After blood samples were pretreated, Propofol concentrations of plasma were determined by high performance liquid chromatography with ultraviolet detection. Results Sparse sampling was used to obtain 285 concentration data at different time points.The data were simple pharmacokinetic analyzed, there was no significant difference between the nations. Conclusion The method provides a sensitive, accurate, precise and reliable analytical procedure for the therapeutic drug monitoring of Propofol in population pharmacokinetic studies. In order to explore the impact of ethnic factors on the pharmacokinetics of Propofol, it needs further research and analysis.