脂联素及脂联素受体1的表达与糖尿病心肌病的关系

Expressions of adiponectin and adiponectin receptor 1 and relationship between them and diabetic cardiomyopathy

目的通过观察2型糖尿病(T2DM)大鼠脂联素(APN)及心肌脂联素受体1的表达情况,探讨其在T2DM心肌病发病过程中的作用。方法建立T2DM大鼠动物模型。采用双抗体夹心酶联免疫法检测血清及心肌APN水平,并采用免疫组化的方法测定心肌脂联素受体1(Adipo R1)的蛋白表达。结果与对照组比较,T2DM组血清和心肌APN水平下降,心肌Adipo R1蛋白的表达水平降低,差异均有统计学意义(P<0.05)。相关性分析显示,T2DM组大鼠血清APN与血清胰岛素、稳态模型胰岛素抵抗指数呈负相关(r=-0.613,P<0.05;r=-0.637,P<0.05),心肌Adipo R1蛋白表达量与心肌APN呈正相关(r=0.89,P<0.05),与HOMA稳态模型(HOMA-IR)及空腹胰岛素水平(FINS)呈负相关(r=-0.697,P<0.05;r=-0.593,P<0.05)。结论糖尿病心肌病大鼠血清及心肌APN水平下降,心肌Adipo R1蛋白表达下降,胰岛素抵抗可能在糖尿病心肌病的发病过程中起到作用。

Objective To discuss the effects of expressions of adiponectin and adiponectin receptor 1 （AdipoR1） in the development of cardiomyopathy through observing them in rats with T2DM. Methods The model of T2DM was established in rats, and the level of serum and myocardial APN was detected by using double-antibody sandwich ELISA, and expression of AdipoR1 protein was detected by using immunohistochemistry technique. Results Compared with control group, the level of serum and myocardial APN decreased, and expression of AdipoR1 protein decreased in T2DM group （P〈0.05）. The correlation analysis showed that serum APN was negatively correlated to serum insulin and HOMA-insulin resistance （HOMA-IR, r=-0.613, P〈0.05;r=-0.637, P〈0.05）. The expression of AdipoR1 protein was positively correlated to myocardial APN （r=0.89, P〈0.05）, and negatively correlated to HOMA-IR and fasting insulin （r=-0.697, P〈0.05; r=-0.593, P〈0.05）. Conclusion The level of serum and myocardial APN, and expression of AdipoR1 protein decrease in rats with diabetic cardiomyopathy. IR may play a role in the development of diabetic cardiomyopathy.