应用RRLC-TOFMS技术快速鉴别中药艾叶中的化学成分

Rapid identification of chemical components in Artemisiae argyi folium by RRLC-TOFMS

目的应用快速液相色谱-高分辨飞行时间质谱（RRLC-TOFMS）技术对中药艾叶中化学成分进行快速鉴别。方法色谱分离采用Agilent Eclipse C18色谱柱（2.1 mm×100 mm,1.8μm）;流动相：乙腈（A）-0.1%甲酸水溶液（B）,梯度洗脱,A相随时间的变化：5%~30%（0~12 min）,30%~80%（12~25 min）,80%（25~30 min）,进样量1μl,流速0.35 ml/min,柱温40℃。TOFMS、电喷雾离子源（ESI）、正离子模式、质量数扫描范围m/z 100~1 500。结果一次性鉴别出艾叶中31种化学成分。结论建立基于RRLC-TOFMS技术对艾叶中化学成分快速鉴别的方法,为中药艾叶的质量控制及体内的深入研究奠定基础。

Objective To identify the chemical components in Artemisiae argyi folium by rapid resolution liquid chromatog-raphy-time of flight mass spectrometry （RRLC-TOFMS）.Methods The separation was performed on an Agilent Eclipse C18 column （2.1 mm×100 mm,1.8 μm ）.The mobile phase consisted of acetonitrile （A） and 0.1% formic acid （B） were in gradient elu-tion.The flowing rate was 0.35 ml/min, the injection volume was 1μl and the temperature of column was 40℃.Time of flight mass spectrometer （ TOFMS） with electro spray ion source （ ESI） was applied to qualitative analysis under the positive ion mode, and mass scan range was m/z 100-1 500 .Results 31 chemical compounds in Artemisiae argyi folium were identified unequivocally .Conclu-sion A rapid and efficient RRLC-TOFMS approach for identifying the chemical constituents of Artemisiae argyi folium had been suc-cessfully established,which paved a way for quality control and further in vivo studies of Artemisiae argyi folium.