急性髓系白血病多药耐药细胞标记蛋白的探讨

Expression of Multidrug Resistance-Associated Proteins in Acute Myeloid Leukemia Cells

目的采用蛋白质组学技术筛查急性髓系白血病(AML)细胞多药耐药标记蛋白。方法将16例AML患者根据化疗疗效的不同分为2组:多药耐药组(7例)和化疗敏感组(9例)。采用双向电泳和质谱分析检测2组之间差异表达的蛋白质。结果双向电泳凝胶图像分析结果显示,2组之间差异显著的蛋白点共9个。与化疗敏感组比较,多药耐药组第41号蛋白点的蛋白表达水平明显升高(P<0.01),第7/8、25和36/37号蛋白点的蛋白表达水平均明显降低(均P<0.01)。2组之间鉴定的4个差异表达蛋白分别为:细丝蛋白A(filamin A,FLNa,第7/8号)、β-肌动蛋白(β-actin,第25号)、纤维蛋白原γ链(fibrinogen gamma chain,第36/37号)和热休克蛋白60(heat shock protein 60,第41号)。结论多药耐药AML细胞中FLNa、β-actin、fibrinogen gamma chain和heat shock protein 60有可能成为预测AML患者耐药的标志物。

Objective To screen multidrug resistance-associate proteins in acute myeloid leuke-mia（AML）cells with proteomics technique.Methods According to the response to chemothera-py,bone marrow samples derived from 16 AML patients were divided into multidrug resistance group（n=7）and chemo-responsive group（n= 9）.The differentially expressed proteins between the two groups were detected with two-dimensional electrophoresis and mass spectrographic anal-ysis.Results Two-dimensional electrophoresis gel image analysis showed that there were nine differentially expressed proteins between the two groups.Compared with chemo-responsive group,the expression of No.41 protein increased but the expression of No.7/8,No.25 and No.36/37 proteins decreased in multidrug resistance group（all P 〈0.01）.Four differentially ex-pressed proteins were identified,including filamin A（No.7/8）,β-actin（No.25）,fibrinogen gamma chain（No.36/37）and heat shock protein 60（HSP60,No.41）.Conclusion Multidrug resistance-associate proteins filamin A,β-actin,fibrinogen gamma chain and HSP60 may be used as markers for predicting drug resistance in patients with AML.