针刺治疗大鼠系膜增生性肾小球肾炎的光、电镜观察

Acupuncture Treatment of Mesangial Proliferative Glomerulonephritis in Rats by Light Microscope and Transmission Electron Microscope

目的:观察针刺对系膜增生性肾小球肾炎(Ms PGN)大鼠肾组织的显微结构与超微结构的病理改变,为针刺治疗Ms PGN提供形态学依据。方法:采用综合免疫方法建立Ms PGN大鼠模型,选取命门、双肾俞、关元、中脘等穴位进行针刺治疗,光学显微镜和透射电子显微镜下观察其肾脏病理学改变。结果:与模型组比较,各针刺组肾小球系膜细胞和基质增生减少;系膜区宽度明显下降(P<0.05,P<0.01),系膜区电子致密物消失;足细胞超微结构损伤程度明显减轻。结论:针刺对Ms PGN大鼠具有一定的治疗作用。

Objective： By observing the pathological changes of microstructure and ultrastructure of acupuncture on mesangial proliferative glomerulonephritis（ Ms PGN） in rats,to investigate the morphological mechanisms of prevention and treatment of Ms PGN. Methods： The mesangial proliferative glomerulonephritis（ Ms PGN） rat models were induced by comprehensive immunization. Acupuncture treatment at Mingmen point,Shuangshenyu points Guanyuan point and Zhongwan point was used for the Ms PGN rats,and the pathological changes were observed in kidney by LM and TEM. Results： Both of the acupuncture groups were compared with the model group. The mesangial cell and matrix were obviously decreased; the mesangial region had narrowed down（ P〈0. 05,P〈0. 01）; mesangial immune deposits disappeared and the damage of feet epithelial cells were reduced.Conclusion： The acupuncture has some therapeutic effect on Ms PGN rats.