冬虫夏草菌丝体对肠道菌群失调小鼠血清VIP及SP的影响

Effect of Cordyceps sinensis mycelium on serum vasoactive intestinal peptide and substance P in mice with intestinal dysbacteriosis

目的观察冬虫夏草菌丝体对抗生素诱导的菌群失调小鼠血清血管活性肠肽(VIP)及P物质(SP)含量的影响。方法 SPF级健康BALB/c小鼠48只,通过自由饮用头孢曲松钠溶液构建肠道菌群失调模型,设正常对照组(正常饮水)、模型组(饮用0.5g/L头孢曲松钠溶液)、自然恢复组(建模成功后将抗生素溶液换为无菌水)及菌丝体治疗组(将抗生素溶液换为无菌水1d后,采用冬虫夏草菌丝体灌胃1周)4组,每组12只。受试小鼠无菌取粪便,应用平板稀释法培养分析各组肠道菌群数的变化,色谱法测各组挥发性脂肪酸含量,ELISA法测血清VIP和SP含量。结果与正常对照组比较,模型组益生菌群数明显减少,挥发性脂肪酸含量明显降低,VIP含量明显降低,SP含量明显升高(P<0.01)。与自然恢复组比较,菌丝体治疗组双歧杆菌、乳酸杆菌数量,挥发性脂肪酸及血清VIP含量均明显升高,而SP含量明显降低(P<0.01或P<0.05)。结论冬虫夏草菌丝体可能通过调节VIP和SP水平来调整菌群失调小鼠肠道内有益菌的比例。

Objective To observe the effect of Cordyceps sinensis mycelium on serum vasoactive intestinal peptide（VIP） and substance P（SP） in mice with dysbacteriosis induced by antibiotics.Methods Forty-eight healthy SPF BALB/c mice were randomly divided into the normal control group（normal drink）,the dysbacteriosis model group（induced by oral administration of 0.5 g/L ceftriaxone sodium）,the natural recovery group（oral sterile water to replace antibiotic after reproduction of dysbacteriosis）,and Cordyceps sinensis mycelium treatment group（treated by intragastric administration of Cordyceps sinensis mycelium）.The feces were collected without contamination,and the change in intestinal bacterial number was observed with the plate dilution method.The volatile fatty acid was detected by chromatography.The serum VIP and SP contents were assayed with enzyme linked immunosorbent assay（ELISA）.Results Compared with the normal control group,the numbers of probiotics,volatile fatty acids and serum VIP significantly decreased in the model group,while the serum SP markedly increased（P〈0.01）.Compared with the natural recovery group,the bacteria number,the quantities of volatile fatty acids and serum VIP significantly increased after the Cordyceps sinensis mycelium treatment,while the serum SP significantly decreased（P〈0.01,P〈0.05）.Conclusion Cordyceps sinensis mycelium may effectively adjust the proportion of the probiotics in the mice with dysbacteriosis,and the mechanism is apparently related to alteration in the VIP and SP.