人软骨糖蛋白39与帕金森病相关性研究

Study on the correlation between YKL-40 and Parkinson's disease

目的探讨人软骨糖蛋白39(YKL-40)在帕金森病诊断中的临床价值。方法酶联免疫吸附试验检测63例帕金森病患者血清YKL-40表达水平,统一帕金森病评价量表(UPDRS)和Hoehn-Yahr分期评价患者日常生活活动能力、运动功能和病情严重程度,Spearman秩相关分析比较YKL-40表达水平与帕金森病之关联性,受试者工作特征(ROC)曲线评价YKL-40诊断帕金森病敏感性和特异性。结果帕金森病组患者血清YKL-40表达水平[(3.72±0.10)ng/ml]高于对照组[(2.92±0.18)ng/ml]且差异有统计学意义(t=4.141,P=0.000)。血清YKL-40表达水平与UPDRS评分(rs=0.872,P=0.000)和Hoehn-Yahr分期(rs=0.704,P=0.000)均呈正相关。ROC曲线显示YKL-40诊断帕金森病之灵敏度为95.26%、特异度73.34%。结论血清YKL-40表达变化与帕金森病病情严重程度有关,可以作为诊断帕金森病的实验室参考指标之一。

Objective To investigate the value of YKL-40 in diagnosing Parkinson＇s disease （PD）. Methods Blood samples of 63 PD patients and 60 healthy controls Were collected in this study. YKL-40 levels were measured by enzyme-linked immunosorbent assay （ELISA）. Unified Parkinson＇s Disease Rating Scale （UPDRS） and Hoehn-Yahr （H-Y） stage were used to assess the ability of daily life, motor funciton and disease severity of PD patients. Then the correlation between YKL-40 and motor symptoms of PD was analyzed by Spearman＇s rank correlation analysis. Receiver operating characteristic （ROC） curve was used to investigate the sensitivity and specificity of YKL-40 in diagnosing PD. Results The YKL-40 levels in PD patients were （3.72 ± 0.10） ng/ml, which Was significantly higher than that in heaithy controls [（2.92 ± 0.18） ng/ml; t = 4.141, P = 0.000]. Spearman analysis indicated that there was positive correlation between YKL-40 and UPDRS scores （rs = 0.872, P = 0.000）, and between YKL-40 and H-Y stage （rs = 0.704, P = 0.000）. ROC curve indicated that the sensitivity and specificity of YKL- 40 in diagnosing PD was 95.26% and 73.34%, respectively. Conclusions There was a correlation between YKL- 40 and the severity of PD, so YKL-40 can be used as a biomarker in diagnosing PD.