凝血因子Ⅱ、Ⅴ基因多态性与脑梗死的关联研究

Relationship between gene polymorphisms of blood coagulation factors( Ⅱ and Ⅴ) and cerebral infarction

目的探讨中国湖南长沙地区汉族人群中凝血因子Ⅱ(FⅡ)、凝血因子Ⅴ(FⅤ)基因单核苷酸多态性与脑梗死之间的关联。方法采用PCR及飞行时间质谱技术对351例确诊为脑梗死的汉族患者(病例组)及417例对照组FⅡ基因rs1799963位点、FⅤ基因rs6025位点进行基因分型。结果病例组FⅡ基因rs1799963位点基因型均为G/G,对照组中基因型为G/G和A/G。病例组与对照组中FⅤ基因rs6025位点基因型均为G/G。对基因型及等位基因频率进行χ2检验示,病例组FⅡ基因rs1799963位点的基因型分布与对照组相比较差异无统计学意义(P>0.05);病例组等位基因频率与对照组相比较差异无统计学意义(P>0.05)。Logistic回归分析示,FⅡ基因rs1799963位点多态性与脑梗死不相关(P>0.05)。结论 FⅡ基因rs1799963位点多态性和FⅤ基因rs6025位点多态性均可能与中国长沙汉族人群脑梗死之间不存在关联。

Objective To study the relationship between gene polymorphisms of blood coagulation factors（ factor Ⅱ,FⅡ; factor Ⅴ,FⅤ） and cerebral infarction in the Han Chinese population from Changsha,Hunan Province,China. Methods Polymerase chain reaction and matrix-assisted laser desorption / ionization time-of-flight mass spectrometry were used for genotyping of FⅡ rs1799963 and FⅤ rs6025 in 351 patients diagnosed with cerebral infarction（ patient group） and 417 healthy individuals undergoing physical examination（ control group）. All participants were Han Chinese selected from the Department of Neurology,Third Xiangya Hospital of Central South University,from June 2011 to July 2012. The distribution of genotypes and allele frequency were analyzed by the χ2test. The relationship between selected gene polymorphisms and cerebral infarction was examined by logistic regression analysis. Results The genotypes of FⅡ rs1799963 were G / G in the patient group and G / G plus G / A in the control group. The genotype of FⅤ rs6025 was G / G in all participants. The distribution of genotypes and allele frequency at FⅡ rs1799963 showed no significant difference between the patient and control groups（ P 0. 05）. The gene polymorphisms of FⅡ rs1799963 had no association with cerebral infarction（ P 0. 05）. Conclusions There may exist no associati. Conclusions on between the gene polymorphisms of blood coagulation factors（ FⅡ rs1799963 and FⅤ rs6025） and cerebral infarction in the Han Chinese population from Changsha.