电针对坐骨神经损伤大鼠背根神经节中神经营养因子-3及其受体TrkC的影响

Investigation of electroacupuncture on NT-3 and Trk C expression in dorsal root ganglia in rats with sciatic nerve injury

目的:观察电针对坐骨神经损伤大鼠行为学、神经生长因子-3及其受体Trk C的影响,探讨电针治疗坐骨神经损伤的生物学机制。方法:建立大鼠坐骨神经夹持损伤模型,观察各组大鼠的行为学改变与背根神经节(DRG)中NT-3与Trk C表达情况。结果:模型组、模型对照组的大鼠行为学检测表明,造模后大鼠的感觉功能显著下降(P<0.05),电针治疗后有显著改善(P<0.05),与正常组无显著差异;电针组的NT-3与Trk C表达显著升高(P<0.05),第20天时模型组与正常组相比,NT-3分泌显著增多(P<0.05)。结论:电针治疗可以通过提高NT-3与Trk C的表达,维持神经元存活,促进受损神经修复,改善坐骨神经损伤大鼠的感觉功能。

Objective：To observe the influence of electroacupuncture on ethology,expressions of neurotrophic factor-3（NT3） and tyrosine kinase receptor type C（TrkC） receptor of NT-3 in dorsal root ganglia（DRG） of rats with sciatic nerve injury,and to investigate the biological mechanisms of electroacupuncture in sciatic nerve injury.Method：Sciatic nerve clamping injury were used as the model.The thermal withdraw latency tests were operated to observe the behavior changes.The level of NT-3 and TrkC in DRG was measured by immunohistochemistry.Result：The sensory function of model group and model control group reduced significantly（P〈0.05）.The score of thermal withdraw latency（PWL） increased significantly after electroacupuncture therapy（P〈0.05）The expressions of NT-3 and TrkC in electroacupuncture group increased significantly compared with other four groups （P〈 0.05）.On the 20th d the expression of NT-3 in model group increased significantly compared with normal group （P 〈 0.05）.Conclusion：Electroacupuncture could promote neuron survival,stimulate the recovery of injured nerve and improve sensory function by increasing the release of NT-3 and TrkC in rats with sciatic nerve injury.