摘要
目的:观察电针对坐骨神经损伤大鼠行为学、神经生长因子-3及其受体Trk C的影响,探讨电针治疗坐骨神经损伤的生物学机制。方法:建立大鼠坐骨神经夹持损伤模型,观察各组大鼠的行为学改变与背根神经节(DRG)中NT-3与Trk C表达情况。结果:模型组、模型对照组的大鼠行为学检测表明,造模后大鼠的感觉功能显著下降(P<0.05),电针治疗后有显著改善(P<0.05),与正常组无显著差异;电针组的NT-3与Trk C表达显著升高(P<0.05),第20天时模型组与正常组相比,NT-3分泌显著增多(P<0.05)。结论:电针治疗可以通过提高NT-3与Trk C的表达,维持神经元存活,促进受损神经修复,改善坐骨神经损伤大鼠的感觉功能。
Objective:To observe the influence of electroacupuncture on ethology,expressions of neurotrophic factor-3(NT3) and tyrosine kinase receptor type C(TrkC) receptor of NT-3 in dorsal root ganglia(DRG) of rats with sciatic nerve injury,and to investigate the biological mechanisms of electroacupuncture in sciatic nerve injury.Method:Sciatic nerve clamping injury were used as the model.The thermal withdraw latency tests were operated to observe the behavior changes.The level of NT-3 and TrkC in DRG was measured by immunohistochemistry.Result:The sensory function of model group and model control group reduced significantly(P〈0.05).The score of thermal withdraw latency(PWL) increased significantly after electroacupuncture therapy(P〈0.05)The expressions of NT-3 and TrkC in electroacupuncture group increased significantly compared with other four groups (P〈 0.05).On the 20th d the expression of NT-3 in model group increased significantly compared with normal group (P 〈 0.05).Conclusion:Electroacupuncture could promote neuron survival,stimulate the recovery of injured nerve and improve sensory function by increasing the release of NT-3 and TrkC in rats with sciatic nerve injury.
出处
《中国康复医学杂志》
CSCD
北大核心
2014年第12期1109-1112,共4页
Chinese Journal of Rehabilitation Medicine
基金
国家自然科学基金资助项目(81373759)
博士点专项科研基金资助项目(20130013110016)
北京市自然科学基金资助项目(7142097)
关键词
电针
坐骨神经损伤
神经营养因子-3
酪氨酸激酶受体
electroacupuncture
sciatic nerve injury
neurotrophic factor-3
tyrosine kinase receptor type C