基于3'RACE的胃癌细胞肿瘤相关基因的APA位点分析

Alternative polyadenylation site analysis of tumor-related genes based on 3'RACE in gastric cancer cells

目的分析胃癌细胞中肿瘤相关基因的APA(alternative polyadenylation)位点变化。方法我们选取肿瘤相关基因HSP90α和SEC11A,利用3'RACE方法在胃癌细胞系MKN45、MKN28和AGS中扩增其mRNA的3'端序列,经过测序,与已知数据库UCSC比对分析其APA位点变化。结果与正常数据库相比,胃癌细胞中HSP90α和SEC11A两个基因均出现新的APA位点,产生含有不同长度的3'UTR(untranslated region)的新mRNA异构体。结论胃癌细胞中HSP90α和SEC11A的APA位点发生变化,产生新的mRNA异构体,为研究肿瘤发生发展提供了新思路。

Objective To analyze the alteration in alternative polyadenylation （APA） sites of tumor-related genes in gastric cancer cells. Methods We used 3＇RACE to capture the APA sites of two tumor-related genes （HSP90αand SEC11A） in gastric cancer cell lines MKN45, MKN28 and AGS, and compared the results with annotated poly（A） sites in UCSC database. Results We found new APA sites in the two tumor-related genes in gastric cancer cells to produce new mRNA isoforms with different 3＇UTRs. Conclusions There are new mRNA isoforms of HSP90αand SEC11A derived from ATA in gastric cancer cells, which provides new insights into the mechanisms of gastric tumorigenesis.