趋化因子受体7在胃癌组织及细胞中的表达及意义

Expression of chemokine receptor CXCR7 in gastric cancer tissues and cell lines

目的探讨趋化因子受体7(CXCR7)在胃癌组织及细胞中的表达及意义。方法收集35例胃癌患者的癌组织和癌旁组织,采用RT-PCR及免疫组化分别检测CXCR7 mRNA和蛋白的表达水平,并结合临床资料进行分析;体外培养5种不同分化程度的胃癌细胞系(HGC-27未分化腺癌;MGC-803低分化粘液腺癌;BGC-823低分化腺癌;SGC-7901中分化腺癌;MKN-28高分化腺癌),采用免疫荧光检测各细胞系中CXCR7的表达水平。结果(1)胃癌组织中CXCR7的mRNA和蛋白表达均明显高于癌旁组织(均为P<0.01);(2)胃癌组织中CXCR7的表达与患者的性别、年龄、吸烟史、Hp感染、肿瘤部位及病理类型等均无明显关系,但有饮酒史的胃癌患者CXCR7的表达高于无饮酒史的患者(P<0.05);(3)CXCR7在5种不同胃癌细胞系表达程度不一,与分化程度无明显关系,其在中分化胃腺癌细胞系SGC-7901表达程度最高。结论 CXCR7在胃癌组织中呈高表达,在胃癌细胞系中表达程度不一,推测其可能在胃癌的发生发展中具有重要的作用。

Objective To investigate the expression status of CXCR7 in gastric cancer tissues and cell lines. Methods The expression status of CXCR7 was detected in 35 primary gastric cancer tissues and matched adjacent tissues by immunohistochemistry and RT-PCR. The correlation of CXCR7 expression with the clinicopathological parameters and risk factors of gastric cancer was analyzed. The expression of CXCR7 in gastric cell lines （HGC-27, MGC-803, BGC-823, SGC-7901 and MKN-28） was also detected by immunofluorescence assay. Results The expression of CXCR7 was significantly higher in gastric cancer tissues than in adjacent tissues （P〈0.01）. CXCR7 expression was not correlated with age, gender, smoking history, Helicobacter pylori infection, tumor location or the pathological type, but showed a higher expression level in patients with a alcohol-drinking history than in those without （P〈0.05）. CXCR7 was expressed with variable intensities in the 5 gastric cancer cell lines without correlation with the degrees of cell differentiation;its expression was the highest in SGC-7901 cells, a moderately differentiated human gastric adenocarcinoma cell line. Conclusions CXCR7 is highly expressed in gastric cancer tissues with variable intensities in 5 gastric cancer cell lines, suggesting its important role in gastric cancer progression.