肥大细胞在婴幼儿血管瘤增殖及消退中的作用

The research about the function of mast cells in proliferation and involution of infantile hemangioma

目的婴幼儿血管瘤是儿童最常见的实体肿瘤之一，该研究通过对婴幼儿血管瘤所有3个时期肥大细胞的标志物类胰蛋白酶和VEGF-A的检测，研究肥大细胞在婴幼儿血管瘤形成及消退中的作用。方法22例增殖期、20例消退期和23例消退后期婴幼儿血管瘤石蜡标本分别通过免疫组织化学染色检测葡萄糖转运蛋白-1、类胰蛋白酶和血管内皮因子-A的表达；通过4’，6’二脒基-2-苯吲哚（DAPI）对细胞核进行染色；观察类胰蛋白酶和VEGF-A表达情况，计算婴幼儿血管瘤所有3个时期类胰蛋白酶阳性细胞数，类胰蛋白酶阳性的肥大细胞表达VEGF-A的比例。结果22例增殖期、20例消退期和23例消退后期婴幼儿血管瘤标本的Glut-1表达均为阳性。类胰蛋白酶表达阳性的肥大细胞位于血管内皮细胞组成的血管间隙组织。增殖期、消退期和消退后期血管瘤切片，每9个视野，类胰蛋白酶阳性细胞数分别为（27．26±4．10）个、（82．20±5．27）个和（44．59±3．07）个，不同时期比较，差异有统计学意义（P〈0．01）。增殖期、消退期和消退后期中的类胰蛋白酶和VEGF-A表达均为阳性的细胞分别占100％、（22．00±1．41）％和（13．00±1．38）％，不同时期比较，差异有统计学意义（P〈0．01）。结论由肥大细胞分泌的VEGF-A在婴幼儿血管瘤增殖过程中起至关重要作用。

Objective To explore the function of mast cells in proliferation and involution of infantile hemangioma. Methods Paraffin-embedded sections of 22 proliferating, 20 involuting and 23 involuted IH lesions were used to investigate the expression of tryptase, VEGF-A and GLUT-1 by immunostaining. The nuclei of all cells were stained by 4＂, 6＂-diamidino-2-phenylindole （DAPI）. The expression of VEGF and tryptase were observed. The tryptase ＋ mast cells and the percent of VEGFA ＋ cells in the whole tryptase ＋ mast cells were calculated in all three phases of IH. Results The expression of Glut-1 in all sections of 22 proliferating, 20 involuting and 23 involuted IH lesions were all positive. Mast cells, identified by their expression of tryptase, were located in the interstitium of vessels composed by vascular endothelial cells. The average numbers of tryptase ＋ cells in each 9 views of proliferating, involuting and involuted IH were 27. 26 - 4. 10, 82. 20 ± 5.27, 44. 59 ± 3. 07, respectively,which difference is obviously （P〈0. 01）. 100%, （22. 00 ± 1.41）%, （13. 00 ± 1.38）% of these tryptase ＋ cells also expressed VEGF, in proliferating, involuting and involuted IH, respectively, which was difference is also obviously （P〈0. 01）. Conclusions VEGF-A which was secreted by the most cells plays a vital role in the process of proliferation of IH.