摘要
RNA 干扰(RNAi ) 是调整基因表示和房间功能的古老的细胞内机制。用 RNAi 的大规模基因 silencing (HTS ) 高产量的屏蔽打开了令人激动的边疆系统地在哺乳动物的房间学习基因功能。这条途径使研究人员能在给定的生物上下文和愿望识别基因功能提供可观的新奇卓见。这里,我们在癌症生物学和病毒学用案例研究考察 RNAi HTS 策略和应用。
RNA interference (RNAi) is an ancient intra-cellular mechanism that regulates gene expression and cell function. Large-scale gene silencing using RNAi highthroughput screening (HTS) has opened an exciting frontier to systematically study gene function in mammalian cells. This approach enables researchers to identify gene function in a given biological context and will provide considerable novel insight. Here, we review RNAi HTS strategies and applications using case studies in cancer biology and virology.
关键词
高通量筛选技术
RNA干扰
病毒学
生物学
应用
癌症
RNAI技术
哺乳动物细胞
RNA interference (RNAi), short interferingRNA (siRNA), short hairpin RNA (shRNA), high-throughputscreening, cancer, virology