HPLC法测定鼻窦炎口服液中阿魏酸的含量

Determination of ferulic acid in Bidouyan Oral Solution by HPLC

目的：高效液相色谱法测定鼻窦炎口服液中阿魏酸的含量。方法色谱柱：岛津 C18柱（5020-39001）；流动相：甲醇-1％醋酸溶液（30：70）；流速：1.0 mL·min-1；检测波长：321 nm；柱温：30℃；进样量：10μL。结果本法可用来测定鼻窦炎口服液中阿魏酸的含量。在10.22~102.20μg·mL-1浓度范围内呈线性关系，平均回收率为98.84％。结论该方法简便、准确，可作为鼻窦炎口服液质量控制的方法。

Objective To determine the content of ferulic acid by HPLC in Bidouyan Oral Solution. Methods The determination was carried out with Shimadzd C18 column. The mobile phase was methanol-1%acetic acid solution（30：70） and the flow rate was 1. 0 mL · min-1 . The column temperature was 30 ℃ and the detective wavelength was 321 nm. Results The content of ferulic acid can be determined by HPLC. The linear concentration range of these compound was 10. 22~102. 20 μg · mL-1 . The average recovery was 98. 84%. Conclusion The method was simple and accurate and can be used to control the quality of Bidouyan Oral Solution.