藁本内酯抑制星形胶质细胞中NF-κB依赖的细胞因子的表达

Ligustilide inhibits NF-κB dependent cytokines upregulation in primary cultured astrocytes

目的观察藁本内酯(LIG)对脂多糖(LPS)诱导的星形胶质细胞中促炎性细胞因子表达的抑制作用。方法将原代培养成功的星形胶质细胞随机分成四组,对照组(C组)、LPS组、LIG组、BAY组。C组未加入任何药物刺激细胞;LPS组加入浓度为1μg/ml的LPS刺激细胞6h;LIG组用浓度为50μM的LIG预孵育细胞30min后,再加入LPS刺激6h;BAY组用浓度为20μM的核转录因子(NF)-κB抑制剂BAY11-7082预孵育细胞30min后,再加入LPS刺激6h。采用Real-time PCR方法检测促炎性细胞因子白细胞介素(IL)-1β、肿瘤细胞坏死因子(TNF)-α、IL-6 mRNA的表达;采用Western blot方法检测pNF-κB p65的表达。结果与LPS组比较,C、LIG和BAY组IL-1β、TNF-α、IL-6mRNA的表达明显减少(P<0.05)。与LPS组比较,C、LIG组pNF-κB p65蛋白的表达明显降低(P<0.05)。结论藁本内酯可通过抑制原代培养的星形胶质细胞中NF-κB的活化来抑制LPS诱导的促炎症相关的细胞因子的表达。

Objective To observe the inhibition effect of ligustilide（LIG）on lipopolysaccharide（LPS）-induced pro-inflammatory cytokines expression in the primary cultured astrocytes.Methods The cultured astrocytes were divided randomly into four groups：control group（group C）,groups LPS,LIG and BAY.Group C didn＇t receive any chemicals treatment;Group LPS was stimulated with LPS 1μg/ml for 6h;group LIG was preincubated with LIG 50μM for 30 min,and then stimulated with LPS for 6h;group BAY was preincubated with BAY11-7082 20μM for 30 min,and then stimulated with LPS for 6h.The IL-1!,TNF-α,IL-6mRNA expression was measured by Real-time PCR. The expression of pNF-κB p65 protein was detected by Western blot.Results Compared with group LPS,the IL-1!,TNF-α,IL-6mRNA expression was obviously decreased in groups C,LIG and BAY（P〈0.05）.Compared with group LPS,the pNF-κB p65 protein expression was decreased in groups C,LIG and BAY（P〈0.05）.Conclusion LIG inhibited the expression of pro-inflammatory cytokines by inhibiting NF-κB activation in the primary cultured astrocytes.