卵黄高磷蛋白提取的工艺研究

研究了以鸡蛋黄为原料,通过分离其它水溶性蛋白,脱除脂类,然后用1.75mol/LNaCl溶液提取卵黄高磷蛋白的工艺条件。探讨了用水量、有机溶剂种类及用量、NaCl溶液浓度对卵黄高磷蛋白收率的影响。经过正交试验,确定了最佳工艺条件,为批量生产提供了依据。

In this article ,the precipitation conditions of extracting phosvitin from egg yolk were discussed .In our experiments,the water soluble proteins except phosvitin were removed first then the lipids were removed and at last ,the phosvitin wasisolated from the resulted granules with NaCl solution.Meanwhile, the affecting factors such as amount of water , species oforgano solvent , amount of solvent, NaCl solution and concentration of were studied.The optimal processing conditions wereobtained by orthogenesis test, which provided a foundation for the scale-up batch process.Key words