FTO基因多态性与儿童青少年肥胖指标的相关性

Association between FTO polymorphisms and obesity among children and adolescents

目的研究肥胖含量和脂肪相关基因（FTO）多态性与青少年肥胖指标的相关性,为儿童青少年肥胖的早期复查与预防工作提供依据。方法选择北京市2 030名7～18岁中小学生,用基质支持的激光释放/电离飞行时间质谱分析（MALDI-TOF MS）方法检测rs62048402多态性,用四引物特异扩增突变分析（ARMS-PCR）方法检测rs9939609多态性。分析2个多态性与体质量指数（BMI）、腰围、腰臀比、腰围身高比的关系。结果对BMI、腰围、腰臀比、腰围身高比进行分年龄、性别的标准正态离差变换后,rs62048402多态性与变量的回归系数B值（标准误）分别为0.093（0.046）,0.094（0.046）,0.087（0.046）,0.102（0.046）;rs9939609多态性与以上变量的回归系数B值（标准误）分别为0.095（0.045）,0.099（0.045）,0.095（0.045）,0.103（0.045）。结论 FTO基因多态性rs62048402和rs9939609与中国儿童青少年BMI、腰围、腰臀比、腰围身高比均存在相关性,且对腰围身高比效应最大。

Objective To study the association between fat mass and obesity associated gene （FTO） polymorphisms, rs62048402 and rs9939609 and obesity-related variable. Methods This study was conducted in 2030 students aged 7-18 years in Beijing. Genotyping of rs62048402 was performed with matrix assisted laser desorption ionization time of flight mass spectrometry （MALDI-TOF-MS） , while rs9939609 was genotyped with tetra-primer amplification refractory mutation system analysis （ ARMS- PCR）. The associations between the two polymorphisms and BMI, waist circumference, waist-hip ratio and waist-height ratio were analyzed. Results After age-and sex-specific Z score transformation for BMI, waist circumference, waist-hip ratio and waist-height ratio, the coefficients （S.E.） between rs62048402 and these indexes were 0.093 （0.046） , 0.094（ 0.046）, 0.087 （0.046） , 0.102 （ 0.046）, respectively. The coefficients （ S.E. ） between rs939609 and these indexes were 0.095（ 0.045 ）, 0.099（ 0.045 ）, 0.095 （ 0.045 ） , 0.103 （ 0.045 ）, respectively. Conclusion FTO polymorphisms are correlated with BMF, waist circumference, waist-hip ratio and waist-height ratio in Chinese children and adolescents. The effects of two polymorphisms on waist-height ratio are larger than those on other measurements.