大豆异黄酮分离与精制工艺研究

本文通过对比试验研究大豆异黄酮制品的分离与精制条件,提出了其工艺路线,即提取液经等电点沉淀、离心与超滤处理,初步去杂,大孔树脂吸附,水洗,50%～70%乙醇洗脱大豆异黄酮,减压浓缩而制得大豆异黄酮制品,制品最终纯度大于65%。采用梯度洗脱可得到更高纯度的大豆异黄酮。

The isolated and purified conditions of soybean isoflavones were studied by contrast examination. Fat, protein andthe other impurity from the ethanol extracts could be cleaned elementarily by combining isoelectric precipitation, centrifugaltionat 2000rpm and ultrafiltration (PLHK 100000). The impurity such as sugars could be eluted by distilled water from themacroporous adsorption resin column and the isoflavones could be eluted by 50%~70% ethanol. Results showed that the yieldof isoflavones was more than 85%, the purification degree in the produce was over 65%.