摘要
应用纯化的Bt1杀虫晶体蛋白作为标准蛋白和免疫抗原,通过抗体-抗原-酶标抗体反应,建立了酶联免疫吸附测定法(ELISA),以定量检测转基因玉米中的Bt1表达蛋白。用建立的ELISA法对4种进口玉米产品进行了测定,实验结果得到了免疫印迹分析的验证,并与进口试剂盒方法的定量分析结果相一致,因而建立的ELISA法具有操作简便、快速特异、定量准确、经济实惠的优点,特别适合于大批量检测,有着良好的应用前景。
Based on the antibody-antigen-enzyme antibody reaction and by using puriftied Bt1 insecticidal crystal protein asboth standard protein and immunity antigen, a quantitative enzyme-linked immunosorbent assay (ELISA) method was estab-lished to detect express Bt1 protein in genetically modified maize.4 imported maize samples were determined by ELISA, Incomparison with the western-blotting & KIT assay method, the results were almost identical. It concluded that the ELISAmethod might be useful for easy,rapid,reliable and effective assay in large samples.
出处
《食品科学》
EI
CAS
CSCD
北大核心
2002年第8期217-221,共5页
Food Science
基金
厦门科技计划资助项目(350222001109)
