猪卵巢细胞中凋亡抑制因子cFLIP在卵巢卵泡和黄体中的表达

Expression of anti-apoptotic protein,cellular FLICE inhibitory protein(cFLIP),in porcine ovaries

采用免疫组织化学法检测猪卵巢中细胞凋亡调控蛋白即细胞内FLICE样抑制蛋白(Cellular FLICE-like inhibitory protein,cFLIP)在猪卵巢卵泡和黄体中的表达水平。结果显示,在健康卵泡和功能性黄体中均能够观察到cFLIP的高度表达,而闭锁卵泡和退化黄体中cFLIP的表达显著减弱。结果表明,cFLIP作为一种细胞凋亡抑制因子,参与猪卵巢中的卵巢闭锁和黄体退化。

To investigate the expression of anti-apoptotic protein,the cellular FLICE inhibitory protein（cFLIP）,and its relationship with apoptosis in porcine ovaries.Immunohistochemical method was used to measure the expression level of cFLIP in the follicles and corpus luteum（CL）in porcine ovaries.The results showed that the cFLIP protein was highly expressed in the healthy follicles and functional CLs,but its expression was significantly decreased in the atretic follicles and regressed CLs.Therefore,it can be inferred that cFLIP played a role in follicular atresia and luteolysis as a cellular anti-apoptotic factor in porcine ovaries.