玉米赤霉烯酮对小鼠睾丸间质细胞线粒体的损伤作用

Toxicity of zearalenone on the mitochondria of mice Leydig cells

将不同作用时间(时间梯度组)及不同质量浓度(质量浓度梯度组)的玉米赤霉烯酮(Zearalenone,ZEA)作用于体外培养的原代小鼠睾丸间质细胞,时间梯度组设0(对照组)、6、12、24h4个观察组,染毒质量浓度为2.5mg/L;质量浓度梯度组设0(对照组)、5、10、20mg/L ZEA 4个观察组,染毒时间为12h。采用流式细胞技术测定线粒体膜电位和透射电子显微镜观察细胞超微结构的方法观测ZEA对睾丸间质细胞线粒体的损伤作用。结果显示,睾丸间质细胞线粒体膜电位2.5mg/L ZEA暴露6h较对照组显著下降(P<0.05),暴露12h及24h较对照组都有极显著下降(P<0.01);ZEA质量浓度为5mg/L组较对照组显著下降(P<0.05),10mg/L组和20mg/L组较对照组都有极显著下降(P<0.01)。细胞超微结构分析显示,线粒体的空泡化和内质网断裂的程度与ZEA的暴露存在时间-效应关系和质量浓度-效应关系。结果表明,ZEA可导致睾丸间质细胞线粒体损伤,这是ZEA抑制睾丸间质细胞分泌睾酮的一个重要原因。

In order to explore the toxicity mechanim of ZEA （zearalenone）inhibiting Leydig cells to secret androgen,the primary Leydig cells were exposed to increasing time （time group） and increasing concentration （concentration group） of ZEA. Leydig cells were exposed to 2. 5 mg/L ZEA for 0 （control group） ,6,12 and 24 h,and to 0（control group） ,5,10 and 20 mg/L ZEA for 12 h in vitro, then determining the mitochondrial membrane potential by flow cytometry and the ul- trastructural damage by transmission electron microscopy to observe the toxicity of ZEA on the mitochondria of mice Leydig cells. In comparison with the control group,the results showed that the mitochondrial membrane potential decreased significantly （P〈0. 05,P〈0.01） after exposed to 2.5 mg/L ZEA for 6,12 and 24 h and exposed to 5,10 and 20 mg/L ZEA for 12 h. The ultra-structural analysis indicated that the vacuolization of mitochondria and the disruption of endoplasmic reticulum have a dramatic time-effeets and concentration-effects relationship with the ZEA. These findings demonstrated that the ZEA could inhibit Leydig cells secreting androgen by disturbing mitochondrial membrane potential and damaging ultrastructure.