鸡源印第安纳沙门菌多重耐药性分析

Analysis of multi-drug resistant of Salmonella Indiana from chicken

采集山东不同地区鸡源沙门菌,根据Kauffmann-White方法测定分离株血清型,采用肉汤微量稀释法测试分离株对16种抗菌药物的敏感性,PCR方法检测10种耐药基因,分析耐药表型和耐药基因之间的关系。结果显示：共鉴定出沙门菌80株,其中印第安纳沙门菌60株。药敏试验证实：60株印第安纳沙门菌对阿莫西林-克拉维酸、头孢唑啉、多黏菌素、氨苄西林、多西环素和甲氧苄啶等16种抗菌药物普遍耐药。88.33%菌株多重耐药分布在12~15耐,未发现3耐以下的菌株。PCR扩增出int1,blaTEM,aac（6’）-Ib-cr,floR,catA1,tetA,strA,cmlA 8种耐药基因。超过90%的菌株携带int1,blaTEM,floR和aac（6’）-Ib-cr耐药基因。上述结果表明,耐药表型及耐药基因的符合呈现相关性。

This paper studied the characteristics of multidrug-resistant Salmonellafrom chicken in parts of Shandong province.Serotype was measured by the method of Kauffmann-White,the sensitivity of the isolates to 16 antimicrobial agents was tested by the microdilution method,the 12 resistance genes were detected by PCR,the relationship was analyzed between resistant phenotype and gene type.The results showed that 60 strains of SalmonellaIndiana were isolated from 80 isolates of Salmonella.Drug susceptibility testing indicated that isolates resisted to 16 antimicrobial drugs such as amoxicillin-clavulanic acid,cefazolin,polymyxin,ampicillin,doxycyclinea and trimethoprim.88.33%isolates were resistant to 12-15 antimicrobial agents,not found isolates resistant to less than 3antimicrobial agents.8resistance genes were characterized by PCR,including int1,blaTEM,aac（6＇）-Ib-cr,floR,catA1,tetA,strA and cmlA.More than 90%strains carried resistance gene int1,blaTEM,floR and aac（6＇）-Ib-cr.These result indicated that resistance genes were relevant to the resistant type of antimicrobial agents.