藻细胞和高岭土的存在对病毒MS2存活的影响

Effects of Algae and Kaolinite Particles on the Survival of Bacteriophage MS2

选用病毒MS2作为水中肠道病毒的指示病毒,高岭土和铜绿微囊藻分别作为无机颗粒物和有机颗粒物,研究颗粒物浓度、pH值、不同价态离子浓度、天然有机物(NOM)等水质条件下,无机(高岭土)、有机(铜绿微囊藻)颗粒物存在对病毒MS2存活的影响.结果表明,无机颗粒物高岭土对病毒MS2的存活无明显影响,但当水体钙硬度(钙离子产生的硬度)较大时,病毒MS2的表观存活量增加1个对数;铜绿微囊藻的存在会导致病毒MS2的存活量降低1个对数左右,但当溶液的pH值大于4.0或铜绿微囊藻的浓度小于1.0×106cells·L-1时,藻类对病毒的生存无明显影响;当水体钙硬度较大时,藻反而会增加病毒MS2的存活对数.因此在高浊水、高藻水中,水的钙硬度增加会使水体中病毒生存能力变强,进而增加饮用水的安全风险.

In this study,Bacteriophage MS2,Kaolinite and Microcystis aeruginosa were selected as model materials for human enteric viruses,inorganic and organic particles,respectively. The influence of the inorganic（Kaolinite） or organic（Microcystis aeruginosa）particles on the survival of MS2 at different conditions,such as particles concentration,pH,ion concentration and natural organic matter（NOM） were studied. The results showed that Kaolinite had no effect on the survival of phage MS2 except that apparent survival of MS2 increased 1 logarithm in higher hardness water. Microcystis aeruginosa addition reduced 1 logarithm of MS2 survival. However,when the pH value was greater than 4. 0 or the concentration of Microcystis aeruginosa was less than 1. 0 × 10^6cells·L^-1,Microcystis aeruginosa addition had no influence on the survival of MS2. In higher hardness water,Microcystis aeruginosa protected MS2 viruses and then increased the survival of MS2. In drinking water,resource containing higher concentration of particles,the survival ability of virus would be enhanced with the increase of the hardness and then elevated the risks of drinking water safety.