低聚壳聚糖抑制肿瘤作用的实验观察

目的:观察低聚壳聚糖(6～9个单糖分子)抑制肿瘤的作用。方法:(1)采用体外肿瘤细胞培养法进行低聚壳聚糖抑制肿瘤活性的体外观察。(2)用移植肿瘤试验(实体瘤,腹水瘤) 对低聚壳聚糖进行抑制肿瘤作用的整体观察。结果:低聚壳聚糖能够抑制体外培养的肿瘤细胞DNA的合成。小鼠连续喂服低聚壳聚糖30d后与对照组比较,中剂量组(280mg/d/kg.bw) 和高剂量组(840mg/d/kg.bw)荷腹水瘤小鼠存活时间显著延长 (p<0.01), 荷实体瘤小鼠瘤重明显降低(p<0.05)。结论:低聚壳聚糖具有一定的抑制肿瘤作用。

Objective:To investigate the suppressive effect of oligochitosan (6-9 chitosan molecules) on ascites tumor andsolid tumor. Methods: (1) By in vitro incubation of ascites tumor cells, investigating the suppressive effect of oligochitosan onthe tumor cell growth .(2) By feeding with different doses of oligochitosan and transplanting cancer cells-solid tumor andascites tumor to the mice respectively, observing lifetime and investigating in vivo suppressive effect of oligochitosan.Results:Oligochitosan could suppress DNA synthesis of the incubated ascites tumor .After feeding oligochitosan for 30 days, thelifetime of the medium dose group (280mg/day/kg.bw) and the high dose group (840mg/day/kg.bw) of the solid tumor trans-planted mice stayed significantly longer incomparison with that of the control mice (p<0.01).And the tumor weight of themice borne solid tumor was significantly lower than that of the control (p<0.05). Conclusion: The oligochitosan could suppressthe ascites tumor and solid tumor to a certain degree.