稀土铕荧光聚合物的制备及与DNA的相互作用

Preparation and DNA Interaction of Cationic Europium Fluorescent Copolymer

含稀土金属的聚合物既保留了稀土金属的独特性质,又具有聚合物的优点,成为材料科学领域研究的热门之一。文中用含铕稀土配合物单体Eu(AA)3Phen、2-甲基丙烯酰氧乙基-三甲基氯化铵、N-异丙基丙烯酰胺成功合成出阳离子型稀土荧光聚合物,并用核磁共振谱、凝胶渗透色谱、荧光光谱、琼脂糖凝胶电泳、Zeta电位及粒径分析、透射电镜等对其结构、性能进行了表征。结果表明,产物结构明确,在594 nm和618 nm处的具有特征荧光发射,与DNA能够通过静电作用相互结合,特征荧光发射强度不断增强,形成的复合物粒径从246.8 nm逐渐增大到579.7 nm;而Zeta电位逐渐从41.2 mV降低到-12.8 mV,并能使DNA在琼脂糖凝胶电泳中的运动受到阻滞。

Rare earth metal containing polymer materials not only retain the unique nature of the rare earth metal ions, but also have characteristics of polymer materials, thus become a hot research field of materials science. In this paper, cationic europium fluorescent copolymer was successfully synthesized using Eu （AA）3 Phen as complex monomer containing rare earth europium, 2-methyl acryloyl oxygen ethyl trimethyl ammonium chloride, N-isopropyl acrylamide and its structure and properties were characterized by 1H-NMR, gel permeation chromatograph （GPC）, fluorescence spectroscopy, agarose gels electrophoresis, zeta potentials, size characterization and TEM. The results show that, the product is well-defined and has characteristic fluorescent emission at 594 nm and 618 nm, which is gradually reinforced after interacting with DNA in a electrostatic mode; the complex size increases gradually from 246.8 nm to 579.7 nm but the zeta potential decreases from 42.1 mV to -12.8 mV; DNA is retained by the product in agarose gels electrophoresis assays.