电子束辐射引发沉淀法制备氯霉素分子印迹微球

Precipitate Preparation of Chloramphenicol Molecularly Imprinted Nanospheres By Electron Beam Radiation

为了能够快速合成大小均一的分子印迹微球,采用电子加速器的高能电子束引发,甲基丙烯酸(MAA)为单体,沉淀聚合制备了氯霉素(CAP)分子印迹微球。紫外、红外光谱和静态氮吸附实验结果显示,CAP和MAA能够形成超分子复合物,辐照引发能使CAP成功印迹在三维网络结构中并且具有较大的比表面积280.25 m2/g。扫描电镜和等温吸附实验考察了辐照剂量对微球微观形态和吸附性能的影响,结果显示,在辐照剂量为20 kGy条件下能快速制备出粒径为300 nm^500 nm的印迹微球,最大吸附量为107.65μmol/g,且对氯霉素表现出明显的选择吸附性。

Preparation of uniform size molecularly imprinted nanospheres （MIPMs） from the electron beam radiation of functional monomer （methacrylic acid） and crosslinking agent （ethylene glycol dimethacrylate） in the presence of chloramphenicol as template. The characterization of MIPMs can be achieved by FT-IR and static nitrogen adsorption. The results indicate the template molecule could be fixed in molecularly imprinted polymer and the largest specific area is 280.25 m2/g. The effect of radiation dose on the morphology and binding capacity of MIPMs were studied by SEM and isothermal adsorption experiments. The results show that MIPMs prepared under the condition of 20 kGy radiation dose exhibit spherical shape and the max adsorption amount of chloramphenicol reaches 107.65 μmol/g. The separation factors are 3.08 and 4.66, respectively.