层-层自组装制备三维蜂窝状醋酸纤维素多孔膜

Fabrication of There-Dimensional Acetate Cellulose Honeycomb Porous Films by Layer-by-Layer Self-Assemble Method

以单一组分醋酸纤维素（CA）为成膜材质,不添加任何添加剂条件下,利用水蒸气辅助法层-层自组装制备了三维蜂窝状CA多孔膜。利用扫描电镜观察了多孔膜形貌;研究了溶剂、环境湿度和浓度等因素对所成多孔膜结构影响。实验结果表明,以二氯甲烷为溶剂,制备得到的孔结构规整、排列紧密;环境湿度由43%增加到91%,孔径大小由（1.36±0.24）μm增加到（3.71±0.18）μm;CA的质量分数为1%~2%有利于规整孔的形成。扫描电镜断面观察发现CA膜内部全部成孔,且内部孔径大小为（1.09±0.13）μm,约为表面孔径大小的一半。利用界面能最小化理论解释了三维蜂窝孔的形成机理。此三维多孔膜有利于细胞的粘附、铺展、分化和增殖,可作为一种良好的组织工程支架材料使用。

There-dimensional honeycomb structure of single component acetate cellulose （CA） films was prepared via water-assisted layer-by-layer self-assembly without any additive. Their pore structure and morphology were characterized by scanning electron microscopy （SEM）. The effects of solvent, relative humidity and concentration, on the pore patterns were investigated. Regular honeycomb porous structure was also formed using dichloromethane as solvent. The pore size of there-dimensional CA honeycomb porous films increases from （ 1.36 ±0.24） tim to （3.71 ±0.18） μm with the relative humidity increasing from 43% to 91%. The concentration of 1% -2% favors formation of more regular honeycomb porous structure. The cross-section observed by SEM shows that inside CA films is porous structure formed with the diameter of （1.09 ±0.13） μm. The mechanism of formation of there- dimensional honeycomb porous was explained by interfacial energy minimize theory. This three-dimensional porous film would be in favour of cell adhesion, spreading, differentiation and proliferation, which is promising for applicatio in tissue engineering scaffold materials.