FGF4在肺癌细胞体内和体外中表达差异的研究

Differential Expression of FGF4 between in vivo and in vitro Lung Cancer Cells

FGF4已经被证明是癌基因,它涉及肿瘤的生长和转移,为了解FGF4的表达与肿瘤微环境的关系。我们利用FGF4抗体通过免疫组化对一名肺癌患者癌旁,癌组织,癌组织小鼠移植瘤,二次移植瘤以及原代培养的细胞爬片进行FGF4检测,探究其表达差异。通过对比癌组织与对照组(癌旁组织),FGF4在癌巢中高表达;同样,移植瘤与二次移植瘤的癌巢中与癌旁组织比较,FGF4表达相对较高;但是将癌组织进行原代培养后,免疫组化检测细胞爬片FGF4,发现仅有5%±0.21%的肿瘤细胞表达FGF4,对照蛋白Cytokine作为肿瘤标记物,则在100%的肿瘤细胞中表达。研究提示免疫组化检测到FGF4在体内和体外表达不同,提示肿瘤细胞FGF4的表达与肿瘤微环境调控密切相关,肿瘤微环境对肿瘤细胞的FGF4的调控有着重要作用。

FGF4 is regarded as an oncogene involved in tumor growth, metastasis. To assess its role in the interaction between tumor and the microenvironment, immunohistochemistry analysis was performed on normal tissue,tumor tissue, tumor Xenografts in mice with FGF4 antibody to examine FGF4 expression in vivo. Results show that FGF4 is up-regulation in carcinoma nest compared with adjacent tissues; Similarly, Two kinds of xenografts displayed also high FGF4 expression whereas lower level was detected in paracancer tissues. Interestingly, in vitro, only5%±0.21% of cultured cancer cell deriving from the same patient expressed high level of FGF4. As contrast, Cytokine was expressed in 100% tumor cells. Taken together, these results suggest that FGF4 in tumor cell might play a role in the interaction with tumor microenvironment.