正交提取双水相体系纯化新楝果核印楝素的工艺研究

Study on separation of azadirachtin from new neem kernels by orthogonal design mode and aqueous two-phase system

采用乙醇-无机盐双水相技术,研究了从国产新楝果核中提取印楝素的工艺.通过单因素实验和正交实验探讨了无机盐的质量浓度、提取时间、料液比对印楝素提取率的影响.实验结果表明:正交提取、双水相体系纯化印楝素的最佳条件是:无机盐浓度为0.62g/mL,提取时间为1.5h,料液比为1∶10(g∶mL),此时印楝素的提取率为1.23%.

The separation of azadirachtin from new neem kernels was studied by alcohol-inorganic salt a-queous two-phase extraction technology.The single factor and orthogonal experiments were done to in-vestigate the varitaion of azadirachtin extraction ratio relating to inorganic salt concentration,extraction time and material-solvent ratio.The results showed that the extraction rate of azadirachtin was 1 .2 3%under the optimum condition that the concentration of inorganic salt was 0.62 g/mL,the extraction time was 1 .5 h,and the material-solvent ratio was 1∶1 0 （g∶mL）.