姜黄素通过caspase依赖的凋亡途径抑制LNCaP细胞生长

Curcumin induces apoptosis in LNCaP cells through caspase-dependent apoptotic pathway

目的探讨姜黄素诱导雄激素依赖性前列腺癌LNCaP细胞凋亡过程中caspase途径的作用。方法应用CCK8法检测不同浓度(10、20、30、40、50、60μmol/L)姜黄素作用LNCaP细胞12、24、48h后的细胞生长抑制情况。应用Annexin V/PI双染法检测不同浓度(10、20、40μmol/L)姜黄素作用LNCaP细胞24h后的细胞凋亡情况。应用Western blot检测姜黄素作用LNCaP细胞24h后Bax、Bcl-2、Cleaved-caspase-3、Cytochrome C凋亡相关蛋白的变化。结果姜黄素对LNCaP细胞的生长有明显的抑制作用,且呈时间-剂量依赖性;流式细胞术结果显示姜黄素能诱导LNCaP细胞凋亡,10、20、40μmol/L姜黄素作用LNCaP细胞24、48h后细胞凋亡率分别为(6.01±0.95)%、(15.46±1.13)%、(26.13±1.56)%和(11.19±1.74)%、(25.80±2.47)%、(38.72±2.89)%,且呈时间-剂量依赖性(P<0.05);同时姜黄素能够降低LNCaP细胞中Bcl-2的表达,上调Bax、Cytochrome C、Cleavedcaspase-3的表达。结论姜黄素能抑制LNCaP细胞的生长,其作用可能是通过线粒体途径诱导其凋亡。

Objective To explore the apoptosis-inducing effects of curcumin on the androgendependent prostate cancer cell line LNCaP cells and its mechanism. Methods Cell viability was analyzed with CCK8 assay.LNCaP cells were treated with various concentrations of curcumin（0,10,20,30,40,50,60μmol/L）for 12 h,24hand 48 h.The effect of curcumin on the apoptosis of LNCaP cells was measured by flow cytometry after treatment.The expression of Bcl-2,Bax,Cytochrome C and Cleaved-caspase-3proteins after treatment with curcumin was analyzed using Western blot. Results Curcumin could effectively inhibit the proliferation of LNCaP cells in dose-and timedependent manner.Flow cytometric analysis displayed that the apoptotic rates were（6.01±0.95）%,（15.46±1.13）% and（26.13±1.56）% for 24 h,（11.19±1.74）%,（25.80±2.47）%and（38.72±2.89）%for 48 h,after treatment with curcumin at 10,20 and 40μmol/L（P〈0.05）.Meanwhile,the expression of Bcl-2protein was significantly decreased,the expression of Bax,Cytochrome C,Cleaved-caspase-3 was increased by curcumin treatment. Conclusions Curcumin had obvious inhibitory effect on the proliferation of LNCaP cells through the caspase dependent mitochondrial apoptotic pathway.