中国泉州、武汉两个地区女性宫颈HPV感染的差异性及其基因型分布

Comparative Research on Infection Rates and Gene Spectrum of Cervical Cells in Women Infected with HPV in Quanzhou and Wuhan Regions of China

目的：比较泉州、武汉两个地区女性宫颈脱落细胞中21种人乳头瘤病毒（HPV）感染的差异性及其基因型分布情况。方法收集2012年11月-2014年1月在福建医科大学附属泉州市第一医院、武汉大学人民医院妇科门诊就诊的10068名妇女的宫颈脱落细胞标本，其中泉州地区6823例，武汉地区3245例，采用核酸分子快速导流杂交分型技术（Hy-briMax）对宫颈脱落细胞进行21种 HPV基因型别的检测，并对其结果进行分析。结果10068例宫颈细胞标本中共检出 HPV感染者2439例，HPV总感染率为24.23％（2439/10068）；其中泉州地区 HPV总感染率为22.61％（1543/6823），武汉地区 HPV总感染率为27.61％（896/3245）；泉州一重感染率为17.28％（1179/6823），武汉一重感染率为19.88％（645/3245）；泉州多重感染率为5.33％（364/6823），武汉多重感染率为7.73％（251/3245）。武汉地区 HPV总的、一重和多重感染率较泉州地区有显著性差异（P 值均＜0.05）。泉州地区的优势型别分别为 HPV-16，HPV-52，HPV-58，HPV-11，HPV-CP8304；武汉地区的优势型别分别为 HPV-16，HPV-52，HPV-58，HPV-33，HPV-18。结论武汉地区HPV总的、一重和多重感染率都明显高于泉州地区（P值均＜0.05），两个地区的优势型别各不相同。

Objective To compare the differences of infect rates and genotype distribution of 21 kinds of human papillomavir-us （HPV）in women cervical cells in Quanzhou and Wuhan regions.Methods Collected gynecological clinic of 10 068 women in cervical cell specimens from November 2012 to January 2014 in Affiliated Quanzhou First Hospital of Fujian Med-ical University and Renmin Hospital of Wuhan University,of which 6 823 cases in Quanzhou,Wuhan region 3 245 cases were detected to identify HPV genotypes by using nucleic acid hybridization technique（HybriMax）.Results 2 439 in 10 068 samples of cervical cells were detected to be infected HPV with overall infection rate of 24.23%（2 439/10 068）.The overall HPV infection rate in Quanzhou was 22.61% （1 543/6 823）,and 27.61% （896/3 245）in Wuhan.The single HPV infection rate in Quanzhou was 17.28% （1 179/6 823）,and 19.88% （645/3 245）in Wuhan.The multiple HPV infection rate in Quanzhou was 5.33% （364/6 823）,and 7.73% （251/3 245）in Wuhan （The values of P〈0.05）.The five most common HPV genetypes in Quanzhou was HPV-16,HPV-52,HPV-58,HPV-11 and HPV-CP8304,respectively,in Wuhan was HPV-16,HPV-52,HPV-58,HPV-33 and HPV-18,respectively.Conclusion The overall,single and multiple HPV infection rates in Wuhan were all markedly higher than those in Quanzhou （the values of P〈0.05）.The most common HPV gene-types in two regions were different.