载脂蛋白E基因多态性与小儿肺炎支原体感染的相关性探讨

Investigation of Correlation between Gene Polymorphism of Apolipoprotein E and Mycoplasma Pneumoniae Infection in Children

目的：探讨儿童外周血清中载脂蛋白 E（apolipoprotein E，ApoE）基因多态性与小儿肺炎支原体感染的相关性。方法收集西安医学院第一附属医院和西安市儿童医院2011年3月-2014年3月住院和门诊筛查肺炎支原体感染患儿和健康体检儿童血清共236例，年龄3-8岁，分两组：肺炎支原体感染组患儿110例和正常对照组儿童126例，采用多重等位基因特异性PCR（multi-AS PCR）技术检测各组中 ApoE的基因多态性。结果 ApoE基因呈多态性且有6种基因型，即3种纯合子（ε2/2，ε3/3，ε4/4）和3种杂合子（ε3/2，ε3/4，ε4/2），其中ε3/2有四条带出现，ε3/3，ε3/4和ε4/2均是出现三条带，ε2/2和ε4/4出现两条带；两组中 ApoE基因型分布以ε3/3多见，对照组为66.7％，感染组为46.4％；小儿肺炎支原体感染组ε3等位基因频率及ε3/3基因型频率较对照组降低（P＜0.05），而ε4等位基因频率及ε4/4基因型频率较对照组升高（P＜0.05）。结论 ApoE基因多态性与小儿肺炎支原体感染的发病存在相关性，ε3可能对小儿肺炎支原体感染具有抵御作用，而ε4可能在小儿肺炎支原体感染的发病中起促进作用。

Objective To study the relationship between gene polymorphism of apolipoprotein E （apolipoproteinE,ApoE）of peripheral blood and Mycoplasma pneumoniae infection in children.Methods Collected 236 cases serum of inpatient and outpatient screening in children with Mycoplasma pneumoniae infection and healthy children between March 2011 and March 2014 in the First Affiliated Hospital of Xi’an Medical University and Xi’an Children’s Hospital,at the age of 3-8 years old,divided into two groups：110 cases of control group and 126 cases of Mycoplasma pneumoniae infection in chil-dren.Used multiple allele-specific PCR （multi-AS PCR）to detect gene polymorphism of ApoE in each group.Results ApoE gene was polymorphic and 6 genotypes：3 homozygous （ε2/2,ε3/3,ε4/4）and 3 heterozygote （ε3/2,ε3/4,ε4/2）.Theε3/2 had four bands,ε3/3,ε3/4 and 4/2 had three bands,ε2/2 andε4/4 had two bands.ε3/3 of ApoE genotype distribution in two groups was the most common,control group was 66.7%,infection group was 46.4%.Allele frequencies ofε3 and genotype frequencies ofε3/3 inMycoplasmapneumoniae infection of children were lower than those in control group （P〈0.05）.But allele frequencies ofε4 and genotype frequency ofε4/4 in Mycoplasma pneumoniae infection of children were increased, which were compared with those in control group （P〈0.05）.Conclusion There were an association between ApoE gene polymorphism and the incidence of Mycoplasma pneumoniae infection in children.Allelesε3 seems to be a protective factor and allelesε4 may contribute to the development of Mycoplasma pneumoniae infection of children.