血清叶酸和维生素B12检测在巨幼细胞性贫血诊疗中的作用

Detection of Serum Folate and Vitamin B1 2 in the Role of Gigantic Young Cell Anemia Diagnosis and Treatment

目的：探讨血清叶酸（FOL）和维生素 B12（Vit B12）水平在巨幼细胞性贫血（MA）诊疗中的作用。方法选取2010年3月-2013年3月，经检查有明确细胞形态学诊断的巨幼细胞性贫血患者50例作为观察组；另选取同期来院进行体检的52例健康人群作为对照组，观察组接受FOL和Vit B12治疗8-15天，用西门子SIEMENS ADVIA Centaur CP全自动化学发光免疫分析仪对观察组治疗前、治疗后及对照组分别进行血清 FOL和 Vit B12水平检测，对所有检测结果进行统计分析。结果观察组治疗前血清 FOL和 Vit B12水平（2.23±0.11 ng/ml和125.21±94.14 pg/ml）明显低于对照组（5.49±3.91 ng/ml和356.77±185.46 pg/ml），差异均有统计学显著性意义（t＝5.89，P＜0.01；t＝7.90，P＜0.01）；观察组治疗后的血清 FOL和 Vit B12水平（4.26±1.83 ng/ml和307.43±139.81 pg/ml）明显高于治疗前（2.23±0.11 ng/ml和125.21±94.14 pg/ml），差异均有统计学显著性意义（t＝7.83，P＜0.01；t＝7.52，P＜0.01）；观察组治疗后的血清FOL和 Vit B12水平（4.26±1.83 ng/ml和307.43±139.81 pg/ml）与对照组（5.49±3.91 ng/ml和356.77±185.46 pg/ml）比较，差异无统计学意义（t＝1.78，P＞0.05；t＝1.51，P＞0.05）。结论血清 FOL和 Vit B12水平检测，可作为巨幼细胞性贫血诊断和治疗效果监测的有效手段。

Objective To explore the serum folic acid （FOL）and vitamin B12 （Vit B12）levels in gigantic young cell anemia （MA）in the diagnosis and treatment effect.Methods Select in March 2010 to March 2013,examined with clear cell mor-phology in the diagnosis of 50 patients with gigantic young cell anemia as observation group.Another selection during the same period to hospital for a medical 52 cases of healthy people as control group,observation group treated with FOL and Vit B1 2 8-1 5 d,with SIEMENS SIEMENS ADVIA Centaur CP automatic chemiluminescence immune analyzer to observe group before and after treatment and the control group was used to detect the serum FOL and Vit B1 2 levels,respectively, for all the test results were analyzed.Results Observation group before treatment serum FOL and Vit B12 levels （2.23± 0.11 ng/ml and 125.21±94.14 pg/ml）significantly lower than the control group （5.49± 3.91 ng/ml and 356.70±185.46 pg/ml）,differences were statistically significant （t=5.89,P〈0.01;t=7.90,P〈0.01）.Observation group after treatment serum FOL and Vit B12 levels （4.26±1.83 ng/ml and 307.43±139.81 pg/ml）were significantly higher than those before treatment （2.23±0.11 ng/ml and 125.21±94.14 pg/ml）,differences were statistically significant （t=7.83,P〈0.01;t=7.52,P〈0.01）.Observation group after treatment serum FOL and Vit B12 levels （4.26 ± 1.83 ng/ml and 307.43 ± 139.81 pg/ml）and control group （5.49±3.91 ng/ml and 356.70±185.46 pg/ml）,there was no statistically significant difference （t=1.78,P〉0.05;t=1.51,P〉0.05）.Conclusion The detection of serum FOL and Vit B12 levels,can be used as a gigantic young cell anemia is the effective means to the diagnosis and treatment effect monitoring.