水蕨(Ceratopteris thalictroides)两条MADS-box基因的克隆与分析

Cloning and analysis of two Ceratopteris thalictroides MADS-box genes

MADS-box转录因子,作为一个大的基因家族,在植物生长发育过程中起重要作用,尤其是作为开花植物花器官形成的主要调控者.该基因家族在揭示进化方面也有着重大意义.为了探究MADS-box基因的进化,需要更多的非开花植物中MADS-box基因的信息.选取水蕨(Ceratopteris thalictroides)作为材料,用RACE方法对其MADS-box基因进行克隆与分析.在水蕨中克隆得到两条MADS-box基因,分别命名为CtMADS1和CtMADS2.分析显示:CtMADS1属于MIKC*分支,而CtMADS2属于MIKCc分支,水蕨的这2条MADS-box基因与开花植物的MADS-box基因有着很近的亲缘关系,现在维管植物最近的共同祖先中至少存在2条不同的花同源异形基因,即MADS-box基因.

MADS-box transcription factors, as a large gene family, play an important role in plant growth and development, espe- cially act as key regulators in controlling the identities of floral organs in flowering plants. They are also significant in the evolu- tionary revelation. In order to understand MADS-box genes, we need more information of MADS-box genes in non flowering plant. MADS-box genes of Ceratopteris thalictroides were selected to clone and analysis by using RACE method. Two MADS-box genes, designated CtMADS1 and CtMADS2 in C, thalictroides, were cloned. Analysis indicates that CtMADS1 is belonged to MIKC＂ clade, while CtMADS2 is belonged to MIKCc-clade. Phylogeny suggests that these two MADS-box genes of C. thalictroides have a close relationship with flowering plants ,the data indicates that at least two different MADS-box genes are homologous to floral ho meotic genes existed in the last common ancestor of contemporary vascular plants.