漆酶催化复合染料酸性黑ATT脱色的研究

Decolorization of the compound dye Acid Black ATT catalyzed by laccase

利用白腐菌Ganoderma lucidum wz-32发酵制备的漆酶对印染工业常用的复合染料酸性黑ATT进行催化脱色.在单因子试验的基础上,通过正交优化试验确定酸性黑ATT的最佳脱色条件为:温度50℃,染料浓度0.1 g·L-1,酶量9 U·mL-1,pH 5.0,最适条件下酸性黑ATT的脱色率达87.35%.同时研究了金属离子、酶抑制剂、脱色助剂对酸性黑ATT脱色的影响,结果表明,Co2+、Fe2+、Mn2+、Ca2+、I-对脱色有抑制作用,10mmol·L-1的浓度使脱色率降低了50%以上;Na+、Cu2+在低浓度时对脱色有一定的促进作用;SDS显著抑制漆酶的催化脱色作用,浓度为10mmol·L-1时脱色率降低了68%;HBT是高效的脱色助剂,可显著提高漆酶的脱色效率.

The compound dye of Acid Black ATT,one of the commonly used dyes in the printing and dyeing industry,was decolorized by laccase that was produced by the fermentation of a white rot fungus Ganoderma lucidum wz-32. Based on the single factor test,the optimal decolorizing conditions for Acid Black ATT was obtained via orthogonal experiment. The optimal temperature was 50 ℃; the optimal dye concentration was 0. 1 g·L-1; the optimal enzyme dosage was 9 U·mL-1,and the optimal pH was 5. 0. The maximum decolorization rate for Acid Black ATT was 87. 35% under the optimal conditions. Moreover,the effects of metal ions,enzyme inhibitors and decolorizing auxiliaries on the decolorization were investigated. Results showed that the decolorization rate decreased by more than 50% in the presence of 10 mmol·L-1Co2＋,Fe2＋,Mn2＋,Ca2＋and I-,respectively; both of Na＋and Cu2＋in low concentration could enhance the decoloration. The catalysis was significantly inhibited by SDS and the decolorization rate decreased by 68% in the presence of 10mmol·L-1SDS. HBT was a highly effective decolorizing auxiliary,which could significantly enhance the decolorization efficiency of laccase.