自组装法制备载有阿奇霉素的聚己内酯纳米微球的研究

Preparation of Poly(ε-caprolactone)-Azithromycin Nanospheres via Self-assembly

在没有表面活性剂存在的条件下,通过微相逆转法,阿奇霉素与聚己内酯自组装形成了载有阿奇霉素的聚己内酯纳米微球。采用动态激光光散射、扫描电镜观察、原子力显微镜观察、紫外分光光度法等手段表征了纳米微球的大小、形态以及包封率和载药量,并探讨了纳米微球的形成机理。研究结果表明,采用微相逆转法可以成功制得高包封率和高载药量的载药纳米微球,且其水分散液在低温下十分稳定。

In the absence of surfactants,azithromycin (Azi)and poly(ε-caprolactone)(PCL)self-assemble into PCL-Azi nanospheres via mini-phase inversion method.Dynamic light scattering,SEM and AFM observations,as well as ultraviolet spectroscopy were used to characterize the size,morphology,encapsulation efficiency (EE )and loading capacity (LC)of PCL-Azi nanospheres.Also,the formation mechanism of the nanospheres was discussed. The results show that PCL-Azi nanospheres with high EE (>90%)and LC (>40%)can be facilely prepared via the mini-phase inversion method,and the water dispersion of the nanospheres was very stable at low temperatures.