化学发光免疫分析技术在孕妇产前ToRCH检测中的临床意义

Clinical significance of CLIA method in prenatal TORCH detection of pregnant women

目的:评估化学发光免疫分析(CLIA)技术在孕妇产前ToRCH检测中的临床意义。方法:采用化学发光免疫分析(CLIA)技术对ToRCH特异Ig M抗体,Ig G抗体进行定量检测。结果:598例产前孕妇弓形虫检测中TOX-Ig M阳性1例(1/601),半月后复查转阴;TOX-Ig G无阳性。CMV-Ig M阳性2例,半月后复查CMV-Ig M下降,CMV-Ig G上升;CMV-Ig G阳性率93.87%(597/636)。RV-Ig M阳性率2.91%(17/584);灰区1.03%(5/584),1例半月后复查保持原有水平,4例RV-Ig M上升,RV-Ig G上升,干扰实验均为低干扰,RV-Ig G阳性率73.29%(428/584)。HSV I+II-Ig M阳性率15.0%(174/1160);HSV I+II-Ig G阳性率98.36%(1141/1160)。结论:应用化学发光免疫分析(CLIA)技术,可直接对产前孕妇特异Ig M抗体和Ig G抗体进行动态定量检测。

Objective：To evaluate the clinical significance of the chemiluminescence immunoassay assay （ CLIA） method in prenatal TORCH detection of pregnant women. Methods： The serum IgM and IgG specific antibodies for TORCH of the pregnant women were measured with CLIA. Results： Among the 598 pregnant women, the TOX-IgM antibody was positive in one case （ 1/601）, and it turned negative two weeks later. All the cases of TOX-IgG antibodies were negative. Two cases of CMV-IgM antibodies were positive in 637 pregnant women and CMV-IgM was reduced and CMV-IgG increased two week later. The positive rates of CMV-IgG and RV-IgM were 93. 87% 597/636） and 2. 91% （17/584）. The rate of Grey area was 1. 03% （5/584） and became normal two weeks later in 1 case. 4 cases of RV-IgM and RV-IgG increased, and interference tests were low interferences. The positive rates of RV-IgG, HSV I＋II-IgM, and HSV I＋II -IgG were 73. 29% （428/584）, 15. 0% （174/1160）, and 98. 36% （1141/1160）. Conclusions：The quantitative analysis of CLIA can directly detect the amount of IgM and IgG of the pregnant women.