活细胞线粒体中SO_3^(2-)/HSO_3^-的荧光成像

Fluorescent Probe for Bisulfite/Sulfite Imaging in Mitochondria

在五甲川菁染料的基础上,合成了一种新型的中位溴取代的五甲川菁染料Cy5-Br,其结构通过质谱和核磁共振波谱表征.性能测试结果表明,该染料作为荧光探针的发射波长可达665 nm,具有较低的细胞毒性,而且对二氧化硫衍生物SO2-3和HSO-3具有很好的选择性.该探针在665 nm处的荧光发射强度随着SO2-3/HSO-3浓度的增加不断降低,具有良好的线性荧光响应.Cy5-Br可以进入活细胞线粒体,对SO2-3和HSO-3的浓度进行实时共聚焦荧光成像.

A new type of pentamethine cyanine dye with bromo group at the central position Cy5-Br was de-signed and synthesized, and the structure is not reported as far as we know. Cy5-Br can identify SO2 deriva-tives bisulfite and sulfite with a good linear response. Furthermore, Cy5-Br has low cytotoxic to living cells, can localize in mitochondria, and image its bisulfite and sulfite ions concentration real-time change in living cells by the laser confocal fluorescence microscope.