烟叶唇柱苣苔叶片分化与植株再生研究

Research on Differentiation and Plant from in vitro Leaf Tissues of Chirita heterotricha Merr.O

以烟叶唇柱苣苔叶片为外植体,MS为基本培养基,研究了植物生长调节剂对其不定芽诱导、增殖与不定根形成的影响,以期建立烟叶唇柱苣苔的再生繁殖体系。结果表明:采用MS+6-BA 0.5mg/L+NAA 0.1mg/L培养基,不定芽诱导率达到100%,该培养基对不定芽的增殖和生长效果好,40d的增殖系数达到7.43;采用MS+NAA 0.5mg/L培养基,30d生根率100%;组培苗在珍珠岩基质中移栽,成活率可达94%以上。

Using the leaves of Chirita heterotricha as explants, MS as basic culture medium, the effect of plant growth regulators on adventitious bud induction,multiplication and root formation were studied in order to establish regeneration reproductive system of Chirita heterotricha Merr. O. The results showed that using the MS＋6-BA 0. 5 mg/L＋ NAA 0. 1 mg/L medium, adventitious bud differentiation rate reached 100 %the medium on shoot proliferation and growth was good,40 days multiplication factor reached 7. 43; MS＋NAA 0. 5 mg/L medium, 30 days rootage rate was 100%the rooted plantlets were transplanted in the pearlite matix and the survival rate was over 94 %.